人胚胎神经干细胞移植治疗脑瘫患儿的临床研究

目的脑瘫是围产期获得性非进行性脑病导致的先天性运动障碍及姿势异常疾病或综合征。国际上脑瘫的发病率为1%～5%,我国脑瘫的发病率为1.8%～4%,每年新增小儿脑瘫患儿40万。脑瘫目前还无有效的治疗手段。近年来,神经干细胞移植治疗脑瘫的研究越来越多,提供了广阔的空间。人胚胎细胞来源的神经干细胞在形态、功能上具备神经元的特性,与宿主神经元突触联系,接受宿主细胞传递的冲动,与患者大脑发生整合,完成再生功能,从根本上治疗脑瘫。方法提取人胚胎脑组织,体外分化、诱导成神经干细胞,将P2-51-5×105-8神经干细胞通过颈动脉或腰穿的方法移植到病人脑内。一个疗程3～4次,间隔1周,1年2-3个疗程。结果临床治疗25例脑瘫病人,好转23例,无效2例。结论人胚胎神经干细胞移植是治疗脑瘫的最有效手段。

Clinical research of Human embryonic stem cell transplantation for treatment of cerebral palsy

Objective:Cerebral palsy is perinatal acquired progressive encephalopathy caused by congenital movement disorders and abnormal postures disease.International cerebral palsy risk for 1%-5%.China′s cerebral palsy risk for 1.8%-4%,An annual increase of 400 000 children patients with cerebral palsy in China.Cerebral palsy have currently no effective treatment methods,In recent years,there are more and more embryonic cells derived neural stem cell for the treatment of cerebral palsy research,provide a broad space to the treatment of cerebral palsy.Embryonic stem cell derived neural stem cells in morphology and function are the same with neuronal properties,With host neurons cell synapses connect,Receiving the impulse of host cell,get together with Patient′s brain,achieve the function of regeneration,Treat cerebral palsy Fundamentally.The purpose of the topic is to discuss the possibility,safety and effectiveness of Human embryonic stem cell transplantation for treatment of cerebral palsy.Methods:Extract of fetal′s brain tissue,In vitro differentiation and induce to the neural stem cells.Transplant the P2-51-5×105-8 neural stem cells to human′s brain with Lumbar puncture puncture or the puncture of carotid artery.3-4times in one course of a treatment,Interval a week,2-3 courses of treatment in one year.Results:There are 25 cases of cerebral palsy patients in clinical treatment,23 cases Improve,2 cases invalid.Conclusions:Transplantation in the treatment of cerebral palsy with Human embryonic neural stem cell is safe and Effective.