咖啡酸对慢性铝过负荷致大鼠脑损伤的保护作用

目的探讨5-LO抑制剂咖啡酸对慢性铝过负荷致大鼠脑损伤的保护作用。方法灌胃给予大鼠葡萄糖酸铝(Al3+200mg.kg-1),1d1次,每周5d,持续20wk,建立慢性铝过负荷致大鼠脑损伤、神经元退变动物模型;5-LO抑制剂咖啡酸(30、10mg.kg-1)分别在每次大鼠铝给予1h后灌胃。以大鼠空间学习记忆能力改变、海马病理形态学变化以及脑组织MAO-B、AChE、SOD活性和MDA含量变化为观察指标。结果铝过负荷明显导致大鼠空间学习记忆能力下降、海马神经元损伤、脑组织MDA含量明显升高、MAO-B和AChE活性明显升高、SOD活性明显降低。给予咖啡酸能明显改善慢性铝过负荷大鼠的学习记忆能力障碍,明显减轻铝过负荷大鼠海马神经细胞的损伤,明显阻遏铝过负荷大鼠海马MDA含量的增加、AChE和MAO-B活性升高以及SOD活性的降低。结论5-LO抑制剂咖啡酸对慢性铝过负荷大鼠脑脑损伤、神经元退变有明显的保护作用。

Effects of caffeic acid on brain damage induced by chronic aluminum overload in rats

Aim To study the protective effect of caffeic acid on brain damage induced by chronic aluminum overload in rats.Methods The brain injury model of rats was established via intragastric administration of aluminum gluconate(element aluminum 200 mg·kg-1) once a day,5 d a week for 20 weeks.The 5-LO inhibitor caffeic acid(30 mg·kg-1,10 mg·kg-1) was intragastrically administered 1 h after aluminum administration.Pathomorphological changes of hippocampal neuron and the spatial learning and memory function were observed.The MAO-B,AChE and SOD activities,and the MDA contents of brain tissue in rats were measured.Results Chronic aluminum overload could markedly induce spatial learning and memory function impairment,and hippocampal neuronal injury in rats.The MAO-B and AChE activities,and the MDA content significantly increased,while SOD activities significantly decreased in chronic aluminum overload rats.The administration of the caffeic acid can significantly prevent rats from learning and memory function impairments and hippocampal neuronal death,and blunted the decrease of SOD activities and increased MDA contents induced by chronic aluminum overload in rats.Caffeic acid also obviously decreased the MAO-B activities and AChE activities of chronic aluminum overload rats.Conclusion Administration of caffeic acid can prevent the rat brain from damage induced by chronic aluminum overload.