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| 葛根素对大鼠心肌缺血再灌注损伤中TLR4的影响 | |
| 引用本文： | 龙训辉,;王齐国,;李颖,;宋云林. 葛根素对大鼠心肌缺血再灌注损伤中TLR4的影响[J]. 广州医学院学报, 2014, 0(6): 30-33 |
| 作者姓名： | 龙训辉, 王齐国, 李颖, 宋云林 |
| 作者单位： | [1]新疆医科大学附属中医医院重症医学科,新疆乌鲁木齐830000; [2]新疆医科大学第一附属医院重症医学科,新疆乌鲁木齐830054 |
| 摘 要： | 目的：探讨葛根素对大鼠心肌缺血再灌注损伤的保护机制。方法：24只雌性Sprague-Dawley （SD）大鼠随机分为假手术组、缺血再灌注组和葛根素干预组，RT-PCR和Western blotting法检测心肌中TLR4 mRNA及TLR4蛋白的表达，同时四唑氮蓝染色测定心肌梗死范围。结果：大鼠心肌缺血再灌注损伤模型造模成功；葛根素干预组的心肌梗死范围明显小于缺血再灌注组（P＜0.01），且该组大鼠TLR4 mRNA及蛋白的表达也显著低于缺血再灌注组（ P＜0.01）。结论：葛根素干预可减轻大鼠心肌缺血再灌注的损伤，其机制可能与抑制TLR4的表达相关。 |
| 关 键 词： | 葛根素 Toll样受体4 心肌缺血再灌注损伤 |
Effects of puerarin on TLR4 in myocardial ischemia-reperfusion injury of rats |
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| Affiliation: | Long Xunhui, Wang Qiguo, Li Ying, Song Yunlin ( 1 Department of Critical Care Medicine, Hospital of Traditional Chinese Medicine Affiliated to Xinfiang Medical University, Urumqi, Xinjiang 830000; 2Department of Critical Care Medicine, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, China) |
| Abstract: | Objective：To investigate the mechanism underlying the protective effects of puerarin on myocardial ischemia-reperfusion injury （ MIRI） in rats. Methods：Twenty-four female Sprague-Dawley （ SD） rats were randomly divided into the sham operation group, ischemia-reperfusion group and puerarin intervention group. The expressions of TLR4 mRNA and protein were assessed by RT-PCR and Western blotting. Meanwhile, the area of myocardial infarction （ MI） was measured by nitroblue tetrazolium （ NBT） staining. Results： The model of MIRI was successfully built in rats. The MI area in the puerarin intervention group was significantly smaller than that in the ischemia-reperfusion group （ P〈0.01） , and the expressions of TLR4 mRNA and protein were significantly lower than those in the ischemia-reperfusion group as well （P〈0.01）. Conclusion： Puerarin intervention can relieve MIRI in rats and its underlying mechanism may be related to the inhibition of TLR4 expression. |
| Keywords: | puerarin Toll-like receptor 4 myocardium ischemia-reperfusion injury |
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