血小板源生长因子对人血管成纤维细胞DNA及胶原蛋白合成的影响

目的:观察血小板源生长因子对培养的人血管成纤维细胞DNA及胶原蛋白合成的影响。方法:采用培养的人血管成纤维细胞,应用 ³H]-TdR和 ³H]-脯氨酸掺入的方法,观察血小板源生长因子-BB对人血管成纤维细胞DNA合成以及胶原蛋白合成的影响。结果:血小板源生长因子-BB可促进静止状态的人血管成纤维细胞DNA及胶原蛋白的合成,在 30μg/L浓度时DNA及胶原蛋白的合成达到高峰,DNA及胶原蛋白分别于 2 4h和 36h合成最为显著。结论:血小板源生长因子-BB可明显促进培养的人血管成纤维细胞DNA及胶原蛋白的合成

Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts

AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using ³H]-TdR incorporation and ³H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L^-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts.