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| 骨髓间充质干细胞在肝部分切除模型小鼠体内向肝细胞分化 | |
| 引用本文： | 吴雄志,赵连三,许倩,张怡,唐红. 骨髓间充质干细胞在肝部分切除模型小鼠体内向肝细胞分化[J]. 生物医学工程学杂志, 2005, 22(6): 1234-1237 |
| 作者姓名： | 吴雄志 赵连三 许倩 张怡 唐红 |
| 作者单位： | 1. 天津医科大学附属肿瘤医院,300060 2. 四川大学,华西医院,感染病中心,生物治疗国家重点实验室,成都,610041 |
| 基金项目： | 教育部博士点基金资助项目(20020610069) |
| 摘 要： | 以含20%胎牛血清的DM EM低糖培养基,原代细胞直接贴壁培养,48 h后换液继续培养,分离BALB/C小鼠的骨髓间充质干细胞,该细胞分为CD44 CD29 与CD44-CD29 两群。将第5代骨髓间充质干细胞注入部分肝切除小鼠肝右叶,术后5 d和14 d分别处死动物,测体重、肝重与肝功,制备肝组织芯片,原位杂交和免疫荧光同步检测Y染色体与肝细胞标记(白蛋白或CK 18),并作图像叠加。发现移植组小鼠在移植后5 d与14 d在注射肝叶与非注射肝叶内皆可检出大量同时表达Y染色体与白蛋白及Y染色体与CK 18的细胞。术后14 d,移植组肝重与肝脏器指数高于模型组。表明骨髓间充质干细胞能在有再生需求的小鼠体内向肝细胞分化并参与再生。 |
| 关 键 词： | 骨髓间充质干细胞 干细胞移植 肝细胞 再生 |
| 收稿时间： | 2004-12-30 |
| 修稿时间： | 2004-12-302005-03-30 |
Differentiation of Bone Marrow Mesenchymal Stem Cells into Hepatocytes in Hepatectomized Mouse |
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| Wu Xiongzhi,Zhao Liansan,Xu Qian,Zhang Yi,Tang Hong. Differentiation of Bone Marrow Mesenchymal Stem Cells into Hepatocytes in Hepatectomized Mouse[J]. Journal of biomedical engineering, 2005, 22(6): 1234-1237 | |
| Authors: | Wu Xiongzhi Zhao Liansan Xu Qian Zhang Yi Tang Hong |
| Affiliation: | National Key Laboratory of Biotherapy, Infectious Diseases Center of West China Hospital of Sichuan University, Chengdu 610041,China |
| Abstract: | CD_(44)~ CD_(29)~ and CD_(44)~-CD_(29)~ bone marrow mesenchymal stem cells(BMSCs) were isolated from bone marrow of BALB/C male donor mouse by adherence selecting in DMEM media with low glucose and 20% fetal bovine serum.Then P5 cells were injected into the liver remnants of hepatectomined female mouse.Simultaneous assays were performed on the injected liver lobe and other liver lobe for detecting the Y-chromosome by fluorescence in situ hybridization and for defecting the albumin or cytokeratin 18 (CK18) by fluorescence immunoassays.Both Y chromosome and albumin(or CK18) positive cell could be detected at 5 days and 14 days after transplantation.At 14 days after transplantation,the liver weight in transplanted mouse was higher than that in model mouse.These results suggest that BMSCs could be induced to differentiate into hepatocytes and participate in the regeneration of liver. |
| Keywords: | Bone mesenchymal stem cells(BMSCs) Stem cell transplant Hepatocyte Regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |