| Fura-2/AM荧光探针法用于大肠杆菌胞内游离钙的测定 |
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| 引用本文： | 卢群,丘泰球.Fura-2/AM荧光探针法用于大肠杆菌胞内游离钙的测定[J].广东药学院学报,2006,22(3):318-319,330. |
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| 作者姓名： | 卢群 丘泰球 |
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| 作者单位： | 1. 广东药学院,生物化学教研室,广东,广州,510006;华南理工大学,广东,广州,510640
2. 华南理工大学,广东,广州,510640 |
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| 摘 要： | 目的探讨经超声处理后大肠杆菌胞内游离钙的变化。方法超声波作用影响大肠杆菌,用Fura-2/AM探针法测定胞内游离钙的变化。结果经超声作用后的大肠杆菌胞内游离钙水平升高,打开了钙依赖性的离子通道,膜两侧离子交流加速,膜通透性增加。结论Fura-2/AM荧光探针法可用于微生物大肠杆菌胞内游离钙的测定。经一定条件下超声作用后的大肠杆菌胞内游离钙水平升高。
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| 关 键 词： | Fura-2/AM 大肠杆菌 荧光探针 游离钙 |
| 文章编号： | 1006-8783（2006）03-0318-02 |
| 收稿时间： | 04 17 2006 12:00AM |
| 修稿时间： | 2006-04-172006-05-05 |
Determined method of fluorescence probe Fura-2/AM for intracellular Ca2+ of Escherichia coli |
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| LU Qun,QIU Tai-qiu.Determined method of fluorescence probe Fura-2/AM for intracellular Ca2+ of Escherichia coli[J].Academic Journal of Guangdong College of Pharmacy,2006,22(3):318-319,330. |
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| Authors: | LU Qun QIU Tai-qiu |
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| Institution: | 1. Guangdong College of Pharmacy, Guangzhou 510006, Guangdong, China; 2. South China University of Technology, Guangzhou 510640, Guangdong, China |
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| Abstract: | Objective Study alteration of intracellular Ca~(2 ) of Escherichia coli(E.coli) by ultrasound treating.Method Determined the alteration of intracellular Ca~(2 ) by Probe Fura-2/AM method after ultrasound treating.Result and conclusion intracellular Ca~(2 ) level increased,opened the ion road of depending on Ca~(2 ),ion transport increased inside or outside and membrane permeability increased.Probe Fura-2/AM method is applied for determining intracellular Ca~(2 ) of Escherichia coli. |
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| Keywords: | Fura-2/AM Escherichia coli(E coli) fluorescence probe Ca~(2 ) |
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