| 硼中子俘获疗法促人黑色素瘤细胞凋亡 |
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| 引用本文： | 孙婷,丁大冬,李斌,陈桂林,韦永新,谢学顺,杨天权,吴庭枫,周幽心,杜子威.硼中子俘获疗法促人黑色素瘤细胞凋亡[J].中华放射医学与防护杂志,2013,33(1):10-13. |
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| 作者姓名： | 孙婷 丁大冬 李斌 陈桂林 韦永新 谢学顺 杨天权 吴庭枫 周幽心 杜子威 |
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| 作者单位： | 215006,苏州大学附属第一医院神经外科暨脑神经研究室 |
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| 基金项目： | 国家自然科学基金(81207142);江苏省医学领军人才与创新团队项目(K201106) |
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| 摘 要： | 目的 研究硼中子俘获疗法(BNCT)体外杀伤人黑色素瘤细胞的效应及机制.方法 首先检测黑色素瘤细胞A375吸收含硼化合物二羟基苯丙氨酸硼(BPA)的情况,然后采用医院中子照射器(IHNI-1)对含硼(10B)细胞进行照射.克隆存活实验检测细胞的放射敏感性,MTT法检测细胞增殖率,流式细胞术检测凋亡,Western blot检测胞质内细胞色素C表达和caspase-9的激活.结果 BPA孵育24 h,A375细胞10B浓度为(2.884±0.148)μg/107个细胞,达到了BNCT杀伤细胞的要求.富含10B的细胞经中子照射2.1 min后存活分数降低为对照组的58％(t=2.964,P＜0.05),细胞经中子照射后24 h增殖率下降为对照组的83％(t=3.286,P＜0.05),BNCT组细胞凋亡率达(55.2±7.9)％,明显高于对照组(t =9.754,P＜0.05),胞质内细胞色素C水平上升且caspase-9激活程度增加(t=7.625、8.307,P＜0.05).结论 BNCT能够杀伤黑色素瘤细胞,其机制可能通过线粒体途径诱导细胞凋亡.
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| 关 键 词： | 硼中子俘获疗法 二羟基苯丙氨酸硼 医院中子照射器 凋亡 |
| 收稿时间： | 2012/5/15 0:00:00 |
Apoptosis of human melanoma cells induced by boron neutron capture therapy |
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| SUN Ting,DING Da-dong,LI Bin,CHEN Gui-lin,WEI Yong-xin,XIE Xue-shun,YANG Tian-quan,WU Ting-feng,ZHOU You-xin and DU Zi-wei.Apoptosis of human melanoma cells induced by boron neutron capture therapy[J].Chinese Journal of Radiological Medicine and Protection,2013,33(1):10-13. |
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| Authors: | SUN Ting DING Da-dong LI Bin CHEN Gui-lin WEI Yong-xin XIE Xue-shun YANG Tian-quan WU Ting-feng ZHOU You-xin and DU Zi-wei |
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| Institution: | Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China |
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| Abstract: | |
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| Keywords: | Boron neutron capture therapy Boronophenylalanine In-hospital neutron irradiator Apoptosis |
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