人嗅黏膜间充质干细胞的生物学特性

目的：观察及研究人嗅黏膜间充质干细胞的生物学特性。方法：分离、培养、鉴定人嗅黏膜间充质干细 胞，在透射电子显微镜、扫描电子显微镜下观察其超微结构，并在体外诱导其向脂细胞、骨细胞、神经干细胞球和神 经元分化。结果：人嗅黏膜间充质干细胞生长以梭形细胞为主，呈放射状排列，高表达表面标志CD73和CD90，不表 达CD34和CD45；在扫描电子显微镜下可见细胞表面有短而粗的微绒毛突起，在透射电子显微镜下可见到两种不同的 细胞形态；具有成脂、成骨、成神经元干细胞球和神经元分化的能力。结论：人嗅黏膜间充质干细胞具有间充质干细 胞的一般生物学特性，经诱导培养后具有多向分化潜能，可作为组织工程修复的理想的种子细胞。

Biological characteristics of human olfactory mucosa mesenchymal stem cells

Objective: To observe the biological characteristics of the human olfactory mucosa mesenchymal stem cells (hOM-MSCs). Methods: The hOM-MSCs were isolated, cultured and identified in vitro. Scanning electron microscope and transmission electron microscope were used to observe the ultrastructure of hOMMSCs. Th e cells were induced towards adipocyte, osteocyte, neural stem cells, neural-like-cells in vitro. Results: The hOM-MSCs were mainly in spindle shape, arranged with radial colony. The hOMMSCs expressed CD73 and CD90 but no CD34 and CD45. Th e short and thick microvilli processes were seen at the surface of hOM-MSCs by scanning electron microscope, and 2 different cellular morphology of hOM-MSCs were seen under transmission electron microscope. Moreover, the hOMMSCs could be differentiated into adipocyte, osteocyte, neural stem cells and neural cells. Conclusion: The hOM-MSCs possess general biological characteristics of MSCs and display multiple differentiation functions. They can be served as ideal seed cells in tissue-engineering for injury repair.