DNA repair synthesis in primary cultures of kidneys from BALB/c and C3H mice treated with dimethylnitrosamine |
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Authors: | G Brambilla M Cavanna P Carlo R Finollo |
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Institution: | Department of Pharmacology, University of Genoa (Italy);Department of Oncology, University of Genoa (Italy) |
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Abstract: | A combined 'in vivo--in vitro' autoradiographic method was employed to examine the DNA repair induced in the kidney by a single dose of dimethylnitrosamine (DMNA). Unscheduled DNA synthesis was found to be dose-dependent in primary kidney cultures of DMNA-treated mice, and practically not detectable in controls. Its amount was positively correlated with the different susceptibility of C3H and BALB/c mice to kidney tumor induction by DMNA. This experimental model appears sensitive and able to provide repeatable results. It may be useful to detect the organotropic activity of a carcinogen toward the kidney. |
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Keywords: | DMEM Dulbecco's modification of Eagle's minimal essential medium DMNA dimethylnitrosamine PBS phosphate buffered saline |
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