聚维酮联合阿霉素体外抑制膀胱癌细胞T24生长并预防膀胱癌患者术后复发

目的探讨聚维酮(PVP)联合阿霉素(ADM)对人膀胱癌细胞株T24的作用及预防膀胱癌术后复发的疗效。方法MTT法检测细胞生长抑制率,流式细胞仪检测细胞周期和黏附作用。对231例浅表膀胱癌患者术后膀胱灌注PVP ADM(实验组)或生理盐水 ADM(对照组),观察预防复发效果。结果2·5%PVP作用24h和72h细胞生长抑制率为15·11%和49·57%;7·5%浓度时,抑制率升为35·42%和79·66%。单用0·25mg/LADM作用48h抑制率为39·05%;合用2·5%和7·5%PVP抑制率升为68·51%和88·39%。5%PVP能使T24细胞的G0/G1期比率下降和G2/M期比率上升。PVP能提高抗鼠IgG1对T24细胞的黏附作用。194例患者获随访,实验组复发率明显降低,复发时间明显延长(均P<0·01)。结论PVP通过阻滞细胞周期G2/M期和增强黏附作用来抑制T24细胞生长,与ADM联合具有协同作用。PVP联合ADM膀胱灌注预防浅表膀胱癌术后复发疗效确切,副作用少。

Polyvinylpyrrolidone combined with Adriamycin inhibits proliferation of bladder cancer cell line T24 and prevents tumor recurrence in bladder cancer patients

Objective To explore the fuction of polyvinylpyrrolidone(PVP) and Adriamycin(ADM) on human bladder cancer cell line T24 and its effects of preventing recurrence of superficial bladder cancer. Methods Cell Suppression rates were treated by MTT assay. Adhesive attraction and cell cycle were measured by flow cytometry. Two hundreds and thirty-three postoperative cases with superficial bladder cancer received intravesical instillation with ADM combined with PVP (experiencial group) or NS (control group). Results Cell Suppression rates were 15.11% and 49.57%, when treated with 2.5% PVP for 24 and 72 h respectively; the rates increased to 35.42% and 79.66%, when treated with 7.5% PVP. Cell Suppression rate for ADM alone was 39.05%, while co-treated with 2.5% and 7.5% PVP for 48 h, the rates increased to 68.51% and 88.39% respectively. 5% PVP significantly blocked the cell cycle progression, decreased G0/G1 phase and increased G2/M phase. Adhesive attraction to T24 cell was significantly increased when treated with 5%PVP. 194 cases were followed up. Compared with control group recurrence rate was clearly decreased (60.0% vs 18.6%, P<0.01) and recurrence time was remarklydelayed (58.8 m vs 20.8 m, P<0.01) in experiencial group. Conclusion PVP can inhibit human bladder cancer T24 cell growth by inducing cell cycle arrest at G2/M phase and improving adhesive action, the effect could be increased by co-treated with ADM. Intravesical instillation of PVP and ADM is effective for preventing tumor recurrence with fewer side effects.