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| 淋球菌膜抗原CppB基因的克隆及在大肠杆菌和减毒鼠伤寒沙门菌中的表达 | |
| 引用本文： | 吴卫,林元凯,江琪,王磊,任大明,谢匡成,温文清. 淋球菌膜抗原CppB基因的克隆及在大肠杆菌和减毒鼠伤寒沙门菌中的表达[J]. 现代免疫学, 1998, 0(1) |
| 作者姓名： | 吴卫 林元凯 江琪 王磊 任大明 谢匡成 温文清 |
| 作者单位： | 复旦大学遗传学研究所遗传工程国家重点实验室，复旦大学遗传学研究所遗传工程国家重点实验室，复旦大学遗传学研究所遗传工程国家重点实验室，复旦大学遗传学研究所遗传工程国家重点实验室，复旦大学遗传学研究所遗传工程国家重点实验室，上海市第一人民医院中心实验室，上海市第一人民医院中心实验室 上海 200433，上海 200433，上海 200433，上海 200433，上海 200433 |
| 摘 要： | 将PCR扩增的淋球菌CppB膜蛋白基因区段,分别克隆在游离蛋白表达载体pKK223-3和融合蛋白表达载体pWR450-1上,以点杂交证实了克隆子。控制合适表达条件,在大肠杆菌中获高效表达,免疫印迹证实其可被淋病患者血清特异识别,将融合蛋白表达质粒转入减毒鼠伤寒沙门氏菌中也获表达,为淋病口服活疫苗的制备奠定了基础。 |
| 关 键 词： | 淋球菌 CppB膜抗原 重组减毒沙门氏菌 口服活疫苗 表达 |
Cloning of the Gonococcal CppB Antigen Gene and its Expression in E. coli and Attenuated S.typhimurium |
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| Wu Wei,Xie Kuangcheng,Lin Yuankai,et al.. Cloning of the Gonococcal CppB Antigen Gene and its Expression in E. coli and Attenuated S.typhimurium[J]. Current Immunology, 1998, 0(1) | |
| Authors: | Wu Wei Xie Kuangcheng Lin Yuankai et al. |
| Abstract: | The gene of a specific antigen protein CppB from 4. 2kb crypid plasmid of Neisseria gon-orrhoeae was cloned by PCR and gene recombinant techniques. Using pure-protein-expressing vector pKK223-3 and fused-protein-expressing vector pWR450-1,the CppB gene was highly expressed in E. coli. Western blot demonstrated its immunological reaction with sera from gonorrhoea patients. Then the fused-protein-expressing plasmid pWRJD was transformed into attenuated Salmonella systems, in which the expression of the fused-protein was also detected. The results might have brought the appearance of gonococal oral live vaccines a step nearer. |
| Keywords: | Neisseria gonorrhoeae CppB antigen gene attenuated Salmonella typhimurium oral live vaccine expression |
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