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| 乙型肝炎病毒基因型分析以及与血清标志物的关系 | |
| 引用本文： | 王利君,袁梁. 乙型肝炎病毒基因型分析以及与血清标志物的关系[J]. 中国实验诊断学, 2009, 13(10): 1393-1394 |
| 作者姓名： | 王利君 袁梁 |
| 作者单位： | 首都医科大学附属北京同仁医院,检验科,北京100730 |
| 摘 要： | 目的乙型肝炎病毒六种主要基因型的分析,并探讨与乙肝血清标志物的关系。方法提取200例北京同仁医院乙型肝炎感染者血清核酸,采用型特异性引物PCR进行基因分型。用电化学发光法检测血清的乙型肝炎病毒表面抗原、表面抗体、e抗原、e抗体、核心抗体。结果B型占26%、C型64%、BC复合型10%;C型的HBeAg阳性率显著性高于B型,在不同的基因型中HBsAg转阴率无显著性差异。结论北方人群中C型占优势,且C型HBV不易发生HBeAg血清学转换。 |
| 关 键 词： | 乙型肝炎病毒 基因型 |
Analysis of hepatitis B virus genotypes and its relationship with serological biomarkers |
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| Affiliation: | WANG Li-jun,YUANG Liang.(Laboratory Department,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China) |
| Abstract: | Objective To analyze hepatitis B virus genotypes and its relationship with serological biomarkers.Methods Serum samples from 200 HBV patients in Beijing Tongren Hospital were tested both genotypes by PCR using type-specific primers and serological biomakers（such as HBsAg／HBsAb／HBeAg／HBeAb／HBcAb） by Electrogenerated Chemiluminescence Assay.Results Of the 200 patients,52 were genotype B,128 were genotype C,and 20 were BC combination.Positive ratio of HBeAg in genotype C was higher than that in genotype B.However,there were no differences in negative ratio of HBsAg among all genotypes.Conclusion Genotype C is the major genotype in northern district.Genotype C is difficult to transfer serological biomarker from HBeAg to HBeAb. |
| Keywords: | hepatitis B virus genotype |
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