前房相关性免疫偏离诱导及预防角膜移植排斥反应实验研究

目的研究前房相关性免疫偏离（ＡＣＡＩＤ）诱导及预防角膜移植排斥反应的作用。方法采用甘氨酸法制备可溶性角膜分类抗原，分组行兔前房注射诱导ＡＣＡＩＤ，并观察各组部分耳膨胀试验和穿透角膜移植术（ＰＫＰ）术后排斥反应。结果角膜上皮、内皮、基质及全角膜可溶性抗原皆可诱导 ＡＣＡＩＤ，诱导质量浓度为 ２． ０ｍｇ／ｍｌ。成功率分别为９０％，１００％，８０％和１００％。同期角膜移植免疫排斥率降低，植片存活时间延长。高危观察组仍可诱导出ＡＣＡＩＤ，并可推迟排斥反应的发生。结论角膜可溶性抗原可以诱导产生ＡＣＡＩＤ，无严重并发症，对兔角膜移植排斥反应具有一定的预防和治疗作用。

Experimental study on induction of anterior chamber associated immune deviation and its preventive function against corneal grafts rejection

Objective To study the induction of anterior chamber associated immune deviation (ACAID ) and its preventive function against corneal grafts rejection. Methods Soluble corneal classified antigens were made by Glycine method and ACAID was induced by injecting glycine into the anterior chamber(AC) of rabbits. The ear swelling was detected to test ACAID induction, and the corneal grafts rejection of each group was observed. Results Corneal epithelium, endothelium, stroma and the whole corneal soluble antigens induced ACAID. The concentration for induction was 2.0 mg/ml, and the ratio for induction was 90%, 100%,80% and 100%, respectively. The rate of rejection was lower than that of the control group and the survival period of donor graft became longer. ACAID was still induced in high risk group and the rejection was delayed. Conclusion Corneal soluble antigen can induce ACAID which prevent from or treat the rejection of corneal grafts. There is no severe complication during the induced process.