帕宁方对帕金森病大鼠行为及氧化应激反应的影响

目的:观察帕宁方对帕金森病(PD)大鼠行为和氧化应激反应的影响.方法:运用6-羟基多巴胺(6-OHDA)立体定向脑内注射复制PD大鼠模型.将PD模型大鼠随机分为模型组、阳性对照组(美多巴0.125 g·kg-1)与帕宁方高、中、低剂量组(按生药量计为84,42,21 g·kg-1),另设同期正常组、假手术组.观察各组治疗前后大鼠旋转圈数,测定大鼠中脑黑质纹状体活性氧(ROS)、丙二醛(MDA)、谷胱甘肽(GSH)含量,谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性.结果:与模型组比较,帕宁方高、中、低剂量组大鼠旋转圈数为(237.26±19.23),(246.71±37.06),(278.81±20.09)圈,活性氧含量为( 231.02±10.11),( 235.87±12.76),(247.93±11.30) U·mg-1,MDA含量为(4.56±1.25),(4.70±1.17),(4.63±1.06) nmol·mg-1,均明显减少；GSH含量明显增多,为(6.20±0.81),(6.24±0.76),(6.18±0.90 )mg·g-1；GSH-Px活性为(2.68±0.27),(2.59±0.34),(2.31±0.22) nU·g-1,SOD活性为(188.40±5.86),( 170.87±9.80),(165.62±6.54)nU·mg-1,均明显增高,差异均有统计学意义(P＜0.05).结论:帕宁方能明显改善PD大鼠旋转行为,减轻其黑质纹状体氧化应激损伤.

Effects of Paning Decoction on Behavior and Oxidative Stress Reaction in Rats with Parkinson's Disease

Objective: To study the effects of Paning decoction on behavior and oxidative stress reaction in rats with Parkinson's disease(PD). Method: The PD model were established through stereotoxic microinjection of 6-OHDA. Fifty SD rats were randomly divided into five groups: model group, positive group(madopar 0.125 g·kg^-1)and the high,middle and low dose of Paning decoction groups(84,42,21 g·kg^-1). In addition, a normal group and a sham operated group were established. The behavior (number of rotation) was observed before and after treatment. Moreover, the contents of reactive oxygen species(ROS), malondialdehyde(MDA) and glutathione(GSH), the activity of glutathione peroxidase(GSH-Px) and superoxidedismutase(SOD) in themed brain nigro-striatum were detected. Result: Compared with the model group, the number of rotation was 237.26±19.23, 246.71±37.06 and 278.81±20.09 (in high, middle and low dosage groups, respectevly). the content of ROS were (231.02±10.11), (235.87±12.76), (247.93±11.30) U·mg^-1, and the content of MDA were (4.56±1.25), (4.70±1.17), (4.63±1.06) nmol·mg^-1, respectevly. The content of ROS and MDA were decreased significantly. On the other hand, the content of GSH were (6.20±0.81), (6.24±0.76), (6.18±0.90) mg·g^-1,the activities of GSH-Px were (2.68±0.27), (2.59±0.34), (2.31±0.22) nU·g^-1, SOD were (188.40±5.86), (170.87±9.80), (165.62±6.54) nU·mg^-1, respectively. The content of GSH and the activity of GSH-Px, SOD were increased significantly. All the experimental data had statistical significance (P<0.05). Conclusion: Paning decoction can improve the behavior in PD rats, furthermore can reduce the injury of oxidative stress.