|
|||||
|
|
| 多房棘球绦虫原头蚴对体外培养宿主肝细胞MAPK信号通路影响的初步研究 | |
| 引用本文： | 张传山,李朝旺,范金亮,李静,吕国栋,王俊华,卢晓梅,温浩,林仁勇. 多房棘球绦虫原头蚴对体外培养宿主肝细胞MAPK信号通路影响的初步研究[J]. 中国病原生物学杂志, 2011, 0(8) |
| 作者姓名： | 张传山 李朝旺 范金亮 李静 吕国栋 王俊华 卢晓梅 温浩 林仁勇 |
| 作者单位： | 新疆医科大学第一附属医院省部共建新疆重大疾病医学国家重点实验室培育基地新疆包虫病基础医学重点实验室; |
| 基金项目： | 国家自然科学基金项目(No.30860253); 新疆包虫病基础医学重点实验室开放课题资助项目(No.XJDX0202-2009-01) |
| 摘 要： | 目的探讨多房棘球绦虫原头蚴对体外培养宿主肝细胞丝裂素活化蛋白激酶(mitogen activated protein kinase,MAPK)信号通路的影响。方法采用宿主大鼠肝细胞BRL3A和人源肝癌细胞系HepG2,分别与多房棘球绦虫原头蚴体外无血清共培养。实验组(与多房棘球绦虫原头蚴共培养)每瓶细胞接种约3×103个原头蚴;处理组(U0126)每瓶细胞先用20μmol/L U0126处理3 h,再接种约3×103个原头蚴,继续共培养,分别在12、24、48、72、96和120 h收集细胞,同时收集对照组(无血清培养基)细胞。利用免疫印迹技术(Western blot)检测p-ERK1/2、p-p38和p-JNK的变化。结果在48 h,肝细胞BRL3A共培养组p-ERK1/2表达活性与对照组相比差异有统计学意义(P<0.05),在12、24和48 h,p-JNK和p-p38表达活性分别有微弱升高;在72和96 h肝细胞HepG2共培养组p-ERK1/2表达活性与对照组相比差异有统计学意义(P<0.05),p-JNK和p-p38表达活性无明显变化;U0126处理组p-ERK1/2活性受抑制。结论多房棘球绦虫原... |
| 关 键 词： | 多房棘球绦虫 原头蚴 肝细胞 MAPK |
Effect of Echinococcus multilocularis protoscoleces on MAPK signaling pathways in cultured hepatic cells in vitro |
|
| ZHANG Chuan-shan,LI Chao-wang,FAN Jin-liang,LI Jing,LV Guo-dong,WANG Jun-hua,LU Xiao-mei,WEN Hao,LIN Ren-yong. Effect of Echinococcus multilocularis protoscoleces on MAPK signaling pathways in cultured hepatic cells in vitro[J]. Journal of Pathogen Biology, 2011, 0(8) | |
| Authors: | ZHANG Chuan-shan LI Chao-wang FAN Jin-liang LI Jing LV Guo-dong WANG Jun-hua LU Xiao-mei WEN Hao LIN Ren-yong |
| Affiliation: | ZHANG Chuan-shan,LI Chao-wang,FAN Jin-liang,LI Jing,LV Guo-dong,WANG Jun-hua,LU Xiao-mei,WEN Hao,LIN Ren-yong(Base to Foster a State Key Lab for Major Disease Research in Xinjiang,Xinjiang Key Laboratory of Echinococcosis,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China) |
| Abstract: | Objective To explore the effect of Echinococcus multilocularis protoscoleces on the activation of host hepatocyte MAPK(mitogen-activated protein kinase) signaling pathways.Methods BRL3A rat hepatocytes and HepG2 human hepatocellular liver carcinoma cells were dispensed into 25 cm2 culture flasks for co-culturing.The experimental group(treated with protoscoleces): BRL3A or HepG2 cells incubated with almost 3 000 protoscoleces for 12,24,48,72,96 and 120 h.The experimental group(treated with U0126): BRL3A or H... |
| Keywords: | Echinococcus.multilocularis protoscolex liver cell MAPK |
| 本文献已被 CNKI 等数据库收录！ | |