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| 正交试验法优选珍珠菜中4种黄酮的提取工艺 | |
| 引用本文： | 丁林芬,马银海,吴兴德,秦智彬,徐文秀,郭亚东.正交试验法优选珍珠菜中4种黄酮的提取工艺[J].昆明医学院学报,2012,33(9):56-58. |
| 作者姓名： | 丁林芬 马银海 吴兴德 秦智彬 徐文秀 郭亚东 |
| 作者单位： | 1. 昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明,650500 2. 昆明学院化学科学与技术系,云南昆明,650214 3. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明,650204 |
| 摘 要： | 目的 优选珍珠菜中芦丁、异槲皮苷、山柰酚3-0-β-D-半乳糖苷及紫云英苷的最佳提取工艺.方法 通过正交试验设计考察甲醇浓度、提取时间、提取温度、料液比对珍珠菜中4种黄酮含量的影响.结果 最佳提取工艺条件为70％甲醇,提取时间45 min,提取温度45℃,料液比(g/mL) 1∶100.结论 该工艺稳定、有效,简单易行. |
| 关 键 词： | 珍珠菜 4种黄酮 正交试验 |
Optimization of Extraction Process of Four Flavonoids in Lysimachia Clethroides Duby by Orthogonal Design |
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| DING Lin-fen , MA Yin-hai , WU Xing-de , QIN Zhi-bin , XU Wen-xiu , GUO Ya-dong.Optimization of Extraction Process of Four Flavonoids in Lysimachia Clethroides Duby by Orthogonal Design[J].Journal of Kunming Medical College,2012,33(9):56-58. | |
| Authors: | DING Lin-fen MA Yin-hai WU Xing-de QIN Zhi-bin XU Wen-xiu GUO Ya-dong |
| Institution: | 1)(1)School of Pharmaceutical Science &Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650500;2)Chemical Science and Technology Department,Kunming University,Kunming Yunnan 650214;3)State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650204,China) |
| Abstract: | Objective To optimize the extraction technology and condition of rutin,isoquercitrin,kaempferol 3-O-β-D-galactoside and astragalin from Lysimachia clethroides Duby.Method The concentration of methanol,the amount of solvent,extraction times and the solid-liquid ratio were investigated by orthogonal design experiment.Result The optimum extract conditions were: 70% methanol as the extraction solvent,45 min of extraction time,45°C of extraction temperature and 1:100 of the solid-liquid ratio.Conclusion The optimum process of four flavonoids is stable,effective and simple. |
| Keywords: | Lysimachia clethroides duby Four flavonoids Orthogonal design |
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