骨髓来源半成熟树突状细胞诱导大鼠小肠移植免疫耐受

目的 观察髓样分化因子88(MyD88)沉默的半成熟树突状细胞(DC)诱导大鼠小肠移植模型免疫耐受.方法 供体乃44大鼠,受体Wistar大鼠.随机分为3组(n=6).A组(半成熟DC组)移植前7d经阴茎背静脉注射半成熟MyD88沉默DC(2×106细胞数),B组(未成熟DC组)移植前7 d经阴茎背静脉注射未成熟DC(2×106细胞数)和C组(阴性对照组)移植前7 d经阴茎背静脉注射1 ml生理盐水.3组均于注射7 d后行大鼠异位节段性小肠移植术,观察统计各组受体存活情况,检测受体血清中的促炎症因子白细胞介素(IL)-2、干扰素(IFN)-γ等的变化.结果 半成熟MyD88沉默DC组[(13.7±1.2)d,n=6]较未成熟DC组[(8.0±1.0)d,n=6,P<0.05]和阴性对照组[(6.0±0.8)d,n=6,P<0.05)存活时间显著延长,同时受体血清中IL-2和IFN-γ等促炎症因子的表达明显降低(P<0.05),病理改变亦较轻微.结论 MyD88沉默的半成熟DC具有独特的表形和功能,能够诱导受体产生特异性免疫耐受,显著延长受体术后的存活时间.

MyD88 silent semi-mature DC induced immune tolerance in rat small bowel transplantation model

Objective To study MyD88 silent semi-mature DC (simDC) inducing immune tolerance in rat small bowel transplantation model. Methods All rats were randomly divided into three groups (n=6). Seven days before transplantation of intestine from F344 donors, Wistar recipient rats were injected with simDC (group A) , imDC (group B) and saline ( group C) through the penile dorsal vein respectively.Small bowel transplantation was performed and the survival time of recipients was observed. Serum IL-2 and IFN-γ levels were assayed. Results The survival time of recipients in group A was ( 13.7±1.2) days, which was significantly longer than that in groups B [(8.0±1.0) days,P<0. 05] and C [(6. 0±0. 8) days P<0. 05]. The serum levels of IL-2 and IFN-γ in group A were lower than in groups B and C (P<0. 05). Conclusion Depending on the unique phenotypic and functional features of sem-DC,they can induce the transplantation tolerance and prolong the survival of intestinal allografts after transplantatin.