| 降脂灵片中决明子定性和定量分析方法的研究 |
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| 引用本文： | 宋玮,杨金荣,张庆伟,张奇,房志仲. 降脂灵片中决明子定性和定量分析方法的研究[J]. 天津医科大学学报, 2001, 7(1): 35-37 |
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| 作者姓名： | 宋玮 杨金荣 张庆伟 张奇 房志仲 |
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| 作者单位： | 1. 天津市塘沽医院,
2. 天津医科大学药学院 |
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| 摘 要： | 目的：通过对决明子的定性和定量分析来控制降脂灵片的质量。方法：采用薄层层析法对决明子进行定性分析；应用高效液相色谱法，采用ODS－C18色谱柱，以甲醇：0．1％高氯酸（80：20）为流动相，检测波长为365nm，测定决明子中大黄素的含量，结果：在0．05－0．8mg/L浓度范围内，线性关系良好，回归方程为Y＝32．92＋5391．86X，r=0.9998，平均回收率为98．70％，结论：本法简便可靠准确，可以作为质控方法。
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| 关 键 词： | 降脂灵片 决明子 大黄素 薄层层析法 高效液相色谱法 中药 化学成分 |
| 文章编号： | 1006-8147(2001)01-0035-03 |
| 修稿时间： | 2000-08-08 |
The study on qualitativeanalysis and quantitative analysis method of Semen Cassiae in JiangZhiLing tablet |
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| SONG Wei,YANG Jin-rong,ZHANG Qing-Wei,ZHANG Qi,FANG Zhi-Zhong. The study on qualitativeanalysis and quantitative analysis method of Semen Cassiae in JiangZhiLing tablet[J]. Journal of Tianjin Medical University, 2001, 7(1): 35-37 |
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| Authors: | SONG Wei YANG Jin-rong ZHANG Qing-Wei ZHANG Qi FANG Zhi-Zhong |
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| Affiliation: | SONG Wei1,YANG Jin-Rong2,ZHANG Qing-Wei2,ZHANG Qi*,FANG Zhi-Zhong2 |
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| Abstract: | Objective :The quality of JiangZhiLing tablet was controlled based on the qualitative analysis and quantitative analysis of Semen Cassiae. Methods: Qualitative analysis of Semen Cassiae was performed by TLC (thin-layer-chromatograph); HPLC(high-performance liquid chromatograph) was applied to determine the quantity of Emodin in Semen Cassiae at 365nm wavelength, in which ODS-C18 volume, 0.1% methanol flow phase and PCA (perchloric acid)were used. Results: Emodin' s detection linear range is 0.05～0.8mg/L. The regression equation is Y=33.92+5391.86X, r=0.9998, average recovery is 98.70%. Conclusion: This method is simple and reliable enough to be used as the method of quality-control. |
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| Keywords: | JiangZhiLing tablet Semen Cassiae Emodin TLC HPLC |
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