P19细胞向心肌样细胞分化前后gp91-phox的变化

目的：探讨诱导P19细胞向心肌样细胞分化前后gp91-phox水平的变化。方法：P19细胞经0.9%二甲基亚砜（DMSO）于细菌培养皿中悬浮诱导培养4 d,待细胞聚集体形成,吸取聚集体接种于组织培养皿,贴壁培养至第13天。行Western blot检测肌钙蛋白I（cTnI）,以鉴定心肌样细胞的分化,并检测P19细胞向心肌样细胞分化前后细胞中gp91-phox蛋白水平。结果：（1）经0.9%DMSO诱导分化,P19细胞于第7天开始表达cTnI,随后cTnI表达明显增高并趋于稳定;（2）P19细胞分化为心肌样细胞后细胞内gp91-phox蛋白水平较分化前高（P〈0.05）。结论：P19细胞向心肌样细胞分化后gp91-phox表达上调,氧化应激水平增加。

Changes of gp91-phox expression before and after the differentiation of P19 ceiis to cardiomyocyte-iike ceiis

Objective：To investigate the changes of gp91-phox expression before and after the differentiation of P19 cells to cardiomyocyte-like cells. Methods：P19 cells were cultured with 0.9% dimethyl sulfoxide（DMSO）in suspension for 4 days to form aggregation.Then the aggregates were transferred to tissue culture dishes for further cultivation up to the 13th day.Cardiac troponin I （cTnI ）was detected by Western blot to identify cell differentiation,then the expression levels of gp91-phox were detected by Western blot before and after the differentiation. Resuits：（1）P19 cells induced by 0.9% DMSO were cTnI-positive at the 7th day,afterwards the expression of cTnI was gradually increased and maintained at a stable level.（2）The expression of gp91-phox in differentiated P19 cells was higher than that in undifferentiated cells （P ＆lt;0.05）. Conciusion：The expression of gp91-phox was up-regulated after the differentiation of P19 cells to cardiomyocyte-like cells indicating the increased oxidative stress level during this process.