肝郁脾虚型肠易激综合征与5-羟色胺转运体基因多态性的关系

[目的]探讨肝郁脾虚型肠易激综合征（IBS）与5-羟色胺转运体（SERT）基因多态性的关系。[方法]用多聚酶链式反应技术（PCR）对50例肝郁脾虚型IBS患者与96例健康对照者SERT基因的启动子区（5-HTTLPER）和内含子2可变数目串联重复序列（VNTRs）多态性进行研究。[结果]肝郁脾虚型组5-HTTLPR基因频率分布是S／S 40．0％，L／S54．0％，L／L11．1％。健康对照组的分布频率是S／S57．3％，L／S35．4％，L／L7．3％。2组之间比较差异无统计学意义（P〉0．05），但肝郁脾虚型组的L／S基因频率比对照组明显升高（P〈0．05）。2组VNTRs区的多态性比较差异无统计学意义（P〉0．05）。[结论]拥有L／S基因型可能是肝郁脾虚型IBS的多个易患因素之一。

Association between irritable bowel syndrome of hepatic stagnation and spleen deficiency type and serotonin transporter gene polymorphism

[Objective] To investigate the relationship between irritable bowel syndrome(IBS) of hepatic stagnation and spleen deficiency type and serotonin transporter(SERT) gene polymorphism.[Methods] Polymerase chain reaction-base technique(PCR) was used to detect the SERT gene-linked region(5-HTTLPER) and the variable number tandem repeats(VNTRs) in intron 2 in 96 healthy subjects and 50 patients with IBS of hepatic stagnation and spleen deficiency type.[Results] The 5-HTTLPER genotypes frequencies showed that S/S was 40.0 %,L/S was 54.0 % and L/L was 11.1 % in the patients,and S/S 57.3 %,L/S 35.4 %,L/L 7.3% in the healthy control.There was no significant difference between two groups(P>0.05).The L/S genotype frequency of 5-HTTLPER alleles was significantly higher in patient group than that in the controls(P<0.05).No significant difference for genotype distribution or the allele frequency of VNTRs was revealed between controls and patient groups(P>0.05).[Conclusion]People with L/S genotype had an increase risk of developing IBS of hepatic stagnation spleen deficiency type.