骨髓单个核细胞对大鼠脊髓损伤后行为功能影响的研究

目的：通过建立大鼠脊髓半横断模型,观察骨髓单个核细胞（BMMNCs）对大鼠后肢功能及受损脊髓组织中神经营养因子3（NT-3）表达的影响.方法：无菌条件下取大鼠双侧股骨和胫骨骨髓细胞,用Ficoll密度梯度离心法分离出单个核细胞备用.将104只Wistar大鼠随机分为A组（假手术组）,8只;B组（PBS对照组）,48只;C组（BMMNCs实验组）,48只.大鼠术后1d、3d、5d、7d、14d和21d行BBB评分后,用免疫组织化学技术观察各组大鼠受损脊髓组织中NT-3的表达.结果：各个时间点BBB评分测试表明A组较B、C组分值高（P＜0.05）;B组分值较C组低（P＜0.05）.免疫组织化学技术检测显示各个时间点C组大鼠受损脊髓中NT-3的表达量高于B组（P＜0.05）,A组与B、C组比较,差异均有统计学意义（P＜0.05）.结论：BMMNCs能够促进损伤脊髓组织中NT-3的表达,可能是其修复损伤脊髓,促进脊髓功能恢复的机制之一.

Effects of BMMNCs on behavioral function in rats with spinal cord injury

Objective：To observe the effects of bone marrow mononuclear cells（BMMNCs） on Hindlimb function of rats and the expression of neurotrophin-3（NT-3） in the injuried Spinal cord of rats with the Rat model of spinal cord hemisection.Methods：We rinse marrow blood from Bilateral femur and tibia under sterile conditions,and isolate BMMNCs in the way of Ficoll density gradient centrifugation.104 Winstar rats were randomly divided into A group（Sham group） with 8 rats; B group（PBS Control group） and C group（BMMNCs Experimental group）,each group of 48.At the 1d,3d,5d,7d,14d and 21d 7d after injury,the rat hindlimb was scored by the Rat BBB Grading,and the expressions of NT-3 were detected by the Immunohistochemistry.Results：At each time point,the BBB scores show that A group is significantly higher than B and C group（P〈0.05）,and B group is less than C group（P〈0.05）.Immunohistochemistry shows that the expressions of NT-3 in the Spinal cord of rats of C group is higher than those in B group（P〈0.05）.The differences between A group and group B and C was statistically significant（P〈0.05）.Conclusion：BMMNCs can promote the expressions of NT-3 in injured spinal cord tissues,which may be one of the mechanisms that BMMNCs repair Spinal cord injury and promote spinal cord function.