冠心病伴抑郁障碍与5-羟色胺转运体基因及纤溶酶原激活物抑制物-1基因多态性关系

目的探讨冠心病伴抑郁障碍与5-羟色胺转运体基因及纤溶酶原激活物抑制物-1基因4G/5G多态性的关系.方法选择对照组63例、单纯抑郁组56例、单纯冠心病组91例和冠心病伴抑郁组75例,采用聚合酶链反应技术检测受试对象5-羟色胺转运体基因上游调控区多态性位点（5-HTILPR）与内含子2区（5-HTIVNTR）及纤溶酶原激活物抑制物-1（PAI-1）基因4G/5G多态性的基因型.结果单纯抑郁组、冠心病伴抑郁组5-HTFLPR的SS基因型（分别为50%和51%）及S等位基因频率（分别为0.69和0.68）明显高于对照组和单纯冠心病组（P＜0.05）;单纯抑郁组、冠心病伴抑郁组LL基因型（分别为13%和15%）及L等位基因频率（分别为0.31和0.32）显著低于对照组和单纯冠心病组（P＜0.05）.单纯冠心病组、冠心病伴抑郁组PAI-1基因4G/4G基因型（分别为47%和48%）及4G等位基因频率（均为0.68）显著高于对照组和单纯抑郁组（P＜0.05）;单纯冠心病组、冠心病伴抑郁组5G/5G基因型（均为12%）及5G等位基因频率（均为0.32）显著低于对照组和单纯抑郁组（P＜0.05）,5-HTIVNTR基因型（12/12,12/10,10/10）分布各组差异无显著性（P＞0.05）,冠心病伴抑郁组SS＋4G/4G联合基因型显著高于对照组、单纯抑郁组和单纯冠心病组（分别为27%、13%、13%和10%,均P＜0.05）.结论5-HTILPR基因SS基因型和PAI-1基因4G/4G基因型共存与冠心病伴抑郁障碍发病相关.

Association between serotonin transporter and plasminogen activator inhibitor-1gene polymorphisms and depressive disorder in patients with coronary heart disease

ObjectiveTo explore the Association between serotonin transporter(5-HTT) and plasminogen activator inhibitor-1(PAI-1)gene polymorphisms and depressive disorder in patients with coronary heart disease(CHD). MethodsUsing a polymerase chain reaction-based technique, polymorphisms of 5-HTT-linked polymorphic region(5-HTTLPR), 5-HTT variable number tandem repeat(5-HTTVNTR) and PAI-1 gene 4G/5G were determined in 75 depressed patients with CHD, 91 non-depressed patients with CHD, 56 depressed patients without CHD and 61 healthy control subjects. ResultsThe frequencies of 5HTTLPR SS genotype and allele S were significantly higher, and the frequencies of LL genotype and allele L were obviously lower in depressed patients with CHD(SS,LL genotype 51%,15%, allele S,L 0.68,0.32,respectively) and depressed patients without CHD(SS,LL genotype 50%,13%, allele S,L 0.69,0.31,respectively) than in control subjects and non-depressed patients with CHD(P<0.05 or 0.01).The frequencies of PAI-1 4G/4G genotype and allele 4G were markedly increased, and the frequencies of 5G/5G genotype and allele 5G were significantly decreased in depressed patients with CHD(4G/4G,5G/5G genotype 48%,12%, allele 4G,5G 0.68,0.32, respectively) and non-depressed patients with CHD(4G/4G,5G/5G genotype 47%,12%, allele 4G,5G 0.68,0.32, respectively) in comparing with control subjects and depressed patients without CHD(P<0.05 or 0.01).No significant difference was found in 5-HTTVNTR genotype distribution among the depressed patients with CHD, non-depressed patients with CHD,depressed patients without DHD and control subjects.There were significant difference in SS +LL combined genotype frequency distribution in depressed patients with CHD(27%) as compared with control subjects(10%),depressed patients without CHD(13%) and non-depressed patients with CHD(13%) (all P< 0.05 ). ConclusionCoexistence of 5-HTTLPR SS and PAI-1 4G/4G genotype in individual could implicate in pathogen of depressive disorder in patients with CHD.