Cytochemical investigation of the alkaline phosphatase activity of the leukocytes after injection of pyrogenal

Summary A cytochemical method of azocombination was used to study the activity of alkaline phosphatase of human leukocytes after injection of pyrogenal (bacterial lipopolysaccharide). Intravenous injection of pyrogenal at first causes a decline and then an increase of the phosphatase activity of neutrophils attaining a maximum on the 3rd–4th day, after which the activity returns to normal. No direct relationship has been discovered between the phosphatase activity of neutrophils and the number of these cells in the peripheral blood. It is presumed that the growth of the phosphatase activity of neutrophils of the peripheral blood is connected with supply of cells rich in alkaline phosphatase from the bone marrow.(Presented by Active Member of the Academy of Medical Sciences of the USSR V. V. Parin). Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 62, No. 10, pp. 44–47, October, 1966.