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| 胸腺五肽一级结构的电喷雾质谱分析 | |
| 引用本文： | 周红华,马仁玲,黄海燕,濮社班,盛龙生. 胸腺五肽一级结构的电喷雾质谱分析[J]. 西北药学杂志, 2004, 19(6): 243-244 |
| 作者姓名： | 周红华 马仁玲 黄海燕 濮社班 盛龙生 |
| 作者单位： | 1. 中国药科大学分析测试中心,江苏,南京,210009 2. 江苏长澳医药有限公司,江苏,南京,210007 3. 中国药科大学中药学院,江苏,南京,210038 |
| 摘 要： | 目的测定胸腺五肽的相对分子质量及其一级结构。方法应用电喷雾质谱法(ESIMS)及源内碰撞诱导解离技术对胸腺五肽进行测定,根据其典型碎片离子确证其氨基酸序列及相对分子质量。结果测得胸腺五肽准分子离子峰(M+H)+m/z680.3及(M+Na)+m/z702.2,(M+2Na-H)+m/z724.3及(M+3Na-2H)+m/z746.2,表明胸腺五肽的相对分子质量为679.4,与理论值一致。结论胸腺五肽的相对分子质量及一级结构与理论值相符。 |
| 关 键 词： | 胸腺五肽 相对分子质量 一级结构 电 喷雾质谱 |
| 文章编号： | 1004-2407(2004)06-0243-02 |
| 修稿时间： | 2004-06-09 |
Determination of the first-order structure of thymopentin(TP5)by electrospray ionization mass spectrometry |
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| ZHOU Hong-hua,MA Ren-ling,HUANG Hai-yan,PU She-ban,SHENG Long-sheng. Determination of the first-order structure of thymopentin(TP5)by electrospray ionization mass spectrometry[J]. Northwest Pharmaceutical Journal, 2004, 19(6): 243-244 | |
| Authors: | ZHOU Hong-hua MA Ren-ling HUANG Hai-yan PU She-ban SHENG Long-sheng |
| Abstract: | Objective To determine the molecular weight and the first-order structure of thymopentin. Methods The molecular weight and amino acid sequence of thymopentin were determined by electrospray ionization mass spectrametry. A series of typical fragment ions were obtained by in-source collision-induced dissociation (CID). The b series of fragment ions were obtained by adjusting the fragmentor voltage. Results The quasi-molecular ions were 680. 3(M + H)+and 702. 2(M+Na)+, 724. 3(M + 2Na-H)+, 746. 2(M + 3Na-2H) + . Conclusion The molecular weight and first-order structure of thymopentin were confirmed. |
| Keywords: | thymopentin molecular weight first-order structure electrospray ionization mass spectrometry |
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