慢性乙型肝炎患者卡波西肉瘤相关疱疹病毒感染状态及特点分析

目的 研究慢性乙型肝炎(乙肝)患者卡波西肉瘤相关疱疹病毒(KSHV)感染状态与HBV复制及治疗因素的关系.方法 ELISA法检测浙江省境内慢性乙肝患者KSHV感染,荧光定量PCR法检测慢性乙肝患者HBV DNA和KSHV DNA,对慢性乙肝患者不矧年龄段、HBV复制状态、甘草类制剂应用等因素进行分析.率的比较行X~2检验.结果 161例男性慢性乙肝患者的KSHV ORF65抗体检出率为27.3%,50例女性慢性乙肝患者为30.0%(X2=0.135,P＞0.05);KSHV感染卒呈现随年龄增加而增高的趋势,40岁后这种趋势不明显,以31～40岁年龄段的KSHV感染率最高,为37.1%.慢性乙肝感染KSHV的患者HBV DNA阳性率为73.5%,高于未感染者的56.3%(X~2=3.969,P＜0.05),其平均HBV DNA比未感染KSHV的慢性乙肝患者高18.6倍.甘草类制剂治疗的慢性乙肝患者血浆KSHV DNA平均水平为204.7拷贝/mL,未用甘草类制剂治疗半均水平为533.9拷贝/mL,如以KSHV DNA＞100拷贝/mL为KSHV DNA阳性,甘草类制剂治疗组16例中有8例阳性,未用甘草类制剂治疗组33例中有23例阳性.结论 KSHV感染率在不同年龄段的慢性乙盯患者呈现随年龄增加而增高的趋势,KSHV感染可能影响HBV的复制,甘草类制剂在体内可能有抑制KSHV复制的作用.

Kaposi's sarcoma-associated herpesvirus infection in chronic hepatitis B patients

Objective To study Kaposi's sarcoma-associated herpesvirus(KSHV)infection in chronic hepatitis B(CHB)patients and its correlation with hepatitis B virus(HBV)replication and treatment-related factors.MethodsEnzyme-linked immunosorbent assay(ELISA)with recombination protein KSHV ORF65 was employed to detect the KSHV antibody and real-time polymerase chain reaction(PCR)was performed to detect KSHV DNA and HBV DNA in CHB patients.Age,HBV replication and licorice preparation treatment of patients were further analyzed.Comparison of rates was done using X~2 test.Results KSHV ORF65 antibody positive rates were 27.3% in 161 male CHB patients and 30.0% in 50 female patients(X~2=0.135,P＞0.05).The KSHV infection rates were increased with age,but this tendency was not obvious in patients older than 40 years old.The highest infection rate was in age group of 31-40 years old which was 37.1%.The positive rate of HBV DNA in CHB patients with KSHV infection was 73.5%,which was 56.3% in uninfected patients(X~2=3.969,P＜0.05).The average plasma level of KSHV DNA in patients treated with licorice preparations was 204.7 copy/mL and that in patients without licorice preparation treatment was 533.9 copy/mL.Eight patients were KSHV DNA positive(KSHV DNA＞ 100 copy/mL)in 16 patients treated with licorice preparations and 23 were positive in 33 patients without licorice preparation treatment.Conclusions The KSHV infection rates are increased with age of CHB patients.KSHV infection may interfere with HBV replication and licorice preparations may suppresss KSHV replication in vivo.