MMP-13在家兔动脉粥样硬化斑块中表达的研究

目的:研究高脂血症家兔的动脉粥样硬化斑块中基质金属蛋白酶-13的表达,为进一步探讨动脉粥样硬化发病机制提供形态学依据.方法:将24只健康雄性新西兰白兔随机分为两组:(1)空白对照组(正常组)8只,每天给予普通饲料;(2)阳性对照组(模型组)16只,每天给予普通饲料 1g胆固醇 15%猪油.16周末所有实验用兔麻醉处死,各组行主动脉粥样硬化病理形态学观察,包括大体形态学观察、光镜观察.根据形态学特点再分成稳定斑块和不稳定斑块,通过免疫组化对斑块内的病变进行定量分析.结果:S-P免疫组化可见模型组中基质金属蛋白酶-13在粥样斑块中平滑肌细胞及泡沫细胞中的细胞核上呈阳性表达,而正常血管壁表达阴性.在稳定性斑块与不稳定性斑块对比中发现,MMP-13在不稳定斑块中表达明显较稳定斑块要高.结论:MMP-13在AS不稳定斑块中较稳定斑块阳性表达明显,显示MMP-13与AS斑块的不稳定性有密切联系.

STUDY ON EXPRESSION OF MATRIX METAL PROTEINASE - 13 ( MMP - 13 ) IN CAROTID PLAQUE

Objective:To investigate the expression of matrix metal propteinase-13(MMP-13)in carotid plaque and to provide the important information for the pathogenesis of atherosclerosis(AS).Methods:24 New Zealand white rabbits were randomly divided into 2 groups : normal group(n=8),model group(n=16).N ormal group always received normal chow;model group always received normal chow,5% cholesterol and 15% lard.At the end of the sixteenth week,all rabbits were killed to performe the pathologic examinations.In addition,the number of grum-positive cell of MMP13 and MMP-2were analyzed by immunohistochemitry showing diseases with atherosclerotic lesion.Results:From this experiment,it thows many MMP-13-positive cells in the fibrous.None in normal group.Conclusion:MMP-13 could express in atherosclerotic plaques in rabbits and has much reliance with AS.