细胞凋亡及bax基因表达与舌苔变化关系的研究

目的:检测常见舌苔舌上皮细胞凋亡情况及凋亡相关基因baxmRNA和蛋白产物,探讨舌苔厚度变化与舌上皮细胞凋亡、bax基因表达的关系。方法:运用TUNEL(末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷三磷酸缺口末端标记)技术、原位杂交、免疫组化和图像分析技术。结果:与正常薄苔比较,剥苔bax基因过度表达伴随细胞凋亡增多,而厚苔bax基因低表达伴随细胞凋亡减少。bax基因表达水平变化趋势与细胞凋亡水平变化趋势一致。结论:bax基因表达水平的变化可能是影响舌苔上皮细胞凋亡并导致舌苔厚度变化的重要原因。

A Study on the Relationship Between the Gene Expression of Bax and Cell Apoptosis in Tongue Mycoderma Epithelium of Common Tongue Coating

Objective: To investigate the relationship between thegene expression of baxand cell apoptosis in tongue mycoderma epithelium of common tongue coating. Methods:TUNEL(terminal deoxynucleotidyl transferase-mediated d-UTP nick-end labeling) technique, in stitu hybridization, immunohistochemical technique and image analysis were used in the study. Results: In comparison with normal tongue coating,there was an over-expression of baxin peeling fur and accompanied with up-regulation of apoptosis ,while there was an lower-expression of baxin thick coated tongue and accompanied with down-regulation of apoptosis.The change ofbaxgene expression level had the same tendency with that of cell apoptosis level. Conclusion:The findings suggested that the change of expression level of baxeffect cell apoptosis in tongue mycoderma epithelium ,thus be one possible cause for the thickness's change of tongue coating.