| 维生素C对人类免疫缺陷病毒灭活作用的研究 |
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| 引用本文： | 樊卫平,张文福,鲍作义,王红霞,李敬云,吕富双.维生素C对人类免疫缺陷病毒灭活作用的研究[J].中国药物与临床,2004,4(7):522-524. |
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| 作者姓名： | 樊卫平 张文福 鲍作义 王红霞 李敬云 吕富双 |
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| 作者单位： | 1. 山西医科大学微生物免疫教研室,030001
2. 军事医学科学院微生物流行病研究所 |
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| 摘 要： | 目的验证维生素C对人类免疫缺陷病毒(HIV)的灭活作用,为生物制品提供一种安全可行的HIV灭活的方法。方法通过测半数组织培养感染剂量(TCID50),检测不同浓度维生素C在不同温度和作用时间下对HIV的灭活作用,并对有效剂量组进行MT4细胞毒性试验。结果500μg/ml维生素C在无血清状态下有灭活HIV作用,而此剂量对MT4细胞有不可逆转的毒性作用。结论维生素C不适宜用于血及血制品的HIV灭活。
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| 关 键 词： | 抗坏血酸 灭活 HIV |
| 修稿时间： | 2004年3月18日 |
The inactivation of HIV by ascorbic acid |
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| FAN Wei-ping,ZHANG Wen-fu,BAO Zuo-yi,WANG Hong-xia,LI Jing-yun,LU Fu-shuang.The inactivation of HIV by ascorbic acid[J].Chinese Remedies & Clinics,2004,4(7):522-524. |
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| Authors: | FAN Wei-ping ZHANG Wen-fu BAO Zuo-yi WANG Hong-xia LI Jing-yun LU Fu-shuang |
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| Institution: | FAN Wei-ping*,ZHANG Wen-fu,BAO Zuo-yi,WANG Hong-xia,LI Jing-yun,LU Fu-shuang. *Department of Microbiology and Immunology,Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective In order to explore the inactivating effect of ascorbic acid on human immunodeficiency virus (HIV) and provide a safe and practical approach to inactivating HIV in biological products. Methods HIV was exposed to different concentrations of ascorbic acid at different temperature. The inactivition result of ascorbic acid on HIV was explored by detecting median tissue culture infective dose (TCID50) at different intervals. The cyto-toxicity of the effective dose on MT4 cell was also explored. Results 500 μg/ml vitamin C can inactivate HIV in serum-free liquid, whereas this dose was toxic to MT4 cell irreversibly. Conclusion ascorbic acid does not suit inactivating HIV in blood or blood products. |
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| Keywords: | HIV |
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