SELDI-TOF-MS蛋白芯片技术在原发性肝癌诊断中的应用研究

目的：筛选AFP阴性原发性肝癌（肝癌）患者与健康人血清中的差异蛋白,构建能与血清AFP联合使用的疾病诊断模型。方法：应用表面增强激光解析—电离飞行时间—质谱（SELDI-TOF-MS）技术和CM10芯片检测33例AFP阴性肝癌患者与35例健康人的血清,获得差异蛋白峰并建立疾病诊断模型。结果：32个蛋白峰差异具有统计学意义（P〈0.05）,在AFP阴性肝癌组血清中有6个上调,26个下调;诊断模型的准确度为80.9%,灵敏度为81.8%,特异度为80.0%。结论：应用SELDI-TOF-MS蛋白芯片技术建立的疾病诊断模型有望与血清AFP联合使用从而提高肝癌的诊断效率。

STUDY ON THE DIAGNOSIS OF PRIMARY HEPATIC CARCINOMA BY SELDI-TOF-MS PROTEIN CHIP TECHNOLOGY

Objective：To detect the serum proteins spectrum of primary hepatic carcinoma（PHC）with AFP-negative and healthy people,and to establish diagnosis models which can be combined with sera AFP.Methods：SELDI-TOF-MS and CM10 Protein Chip technology were used to analyze the serum proteins spectrum in 33 cases of AFP-negative PHC and 35 cases of healthy people.Differential expressed proteins peaks were screened and diagnosis model was established.Results：Thirty-two differential expressed protein peaks were screened（P0.05）,with 6 up-regulated and 26 down-regulated.Diagnosis model was established with an accuracy of 80.9%,sensitivity of 81.8%,and specificity of 80.0%.Conclusion：The diagnosis model established by SELDI-TOF-MS can improve the diagnosis efficiency of PHC in combination with sera AFP.