组织蛋白酶-D在喉鳞状细胞癌中的表达及其意义

目的探讨组织蛋白酶-D（Cathepsin D，Cath-D）在喉鳞状细胞癌侵袭转移中的作用，旨在初步明确其在喉鳞状细胞癌淋巴结转移判断中的作用。方法应用免疫组化SP法检测66例喉鳞状细胞癌标本中Cath—D的表达情况，以其癌旁正常黏膜作为对照，并分析比较与喉鳞状细胞癌患者临床分期、病理分级、淋巴结转移等方面的关系，探讨其临床意义。结果Cath—D在喉鳞状细胞癌组织中阳性表达率为78．79％，在癌旁正常黏膜中是9．09％，两者比较差异有统计学意叉（P〈0．05）。Cath—D阳性表达率在喉鳞状细胞癌患者的年龄、临床分期、临床分型以及淋巴结转移上差异有统计学意义（P〈0．05），而在病理分级方面则没有差异（P〉0．05）。结论Cath-D可能是喉鳞状细胞癌侵袭转移机制中的重要肿瘤标志物，它对于喉鳞状细胞癌淋巴结转移的判断具有重要意义。

Expression and Clinical Significance of Cathepsin-D in Laryngeal Squamous Cell Carcinoma

Objective To investigate the effect of Cathepsin D（Cath-D） on the invasion,metastasis and prognosis of laryngeal squamous cell carcinoma. Methods The expression of Cath-D was detected in 66 cases of laryngeal squamous cell carcinoma and normal mucous membrane as controls by immunohistochemistry （SP method）,and it＇s relationship with the clinical stage, clinical classification, pathological grade and lymph node metastasis of laryngeal carcinoma patients was analyzed. Results The positive rates of Cath-D in laryngeal carcinoma and normal mucous membrane were 78.79% （52/66） and 9.09% （6/66） respectively,the difference was significant between them（ P 〈 0.05 ）. The positive rate of Cath-D has significant difference in patients＇ age, clinical stage, clinical classification and lymph node metastasis （ P 〈 0.05 ）. In addition, the negative rate of Cath-D in laryngeal carcinoma has no relation with pathological grade. Conclusions Cath-D may be important tumour marker. It may play important role during invasion,metastasis and prognosis of laryngeal carcinoma.