应用压电石英晶体生物传感器研究赤芍801与ET-1间的相互作用 |
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引用本文: | 李静,吴宝艳,蒋跃绒,殷惠军,黄加栋,陈可冀,陈强.应用压电石英晶体生物传感器研究赤芍801与ET-1间的相互作用[J].中国中西医结合杂志,2004,24(8):714-716. |
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作者姓名: | 李静 吴宝艳 蒋跃绒 殷惠军 黄加栋 陈可冀 陈强 |
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作者单位: | 1. 南开大学生命科学院,天津,300071 2. 中国中医研究院西苑医院 |
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基金项目: | 国家自然科学基金资助项目 (No .90 2 0 90 54) |
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摘 要: | 目的 采用体外实验的方法,直接检测活血化瘀中药赤芍有效成分赤芍801与细胞因子内皮素-1(ET-1)间的相互作用,探讨其作用的可能的靶点和分子机理。方法 应用压电石英晶体生物传感器——石英晶体微天平(QCM)检测赤芍801与ET-1之间的特异性结合。在QCM金基片表面经活化后接合上亲和素,继而将生物素衍生化的ET-1固定于基片表面,加入赤芍801,检测赤芍801与ET-1之间的相互作用。每-步结合反应后,均用磷酸盐缓冲液(PBS)冲洗以去除非特异性吸附。结果 赤芍801与ET-1发生特异性结合。结论 ET-1可能是赤芍801体内作用靶点之-,即赤芍801通过与ET-1结合,阻碍ET-1与其受体间的结合,从而拮抗ET-1的作用,扩张血管,抑制血小板聚集。
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关 键 词: | 压电石英晶体 生物传感器 赤芍801 ET-1 石英晶体微天平 内皮素-1 |
修稿时间: | 2004年4月30日 |
Study on Interaction of Radix Paeoniae 801 and endothelin-1 by Using a Piezoelectric Quartz Crystal Biosensor |
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Authors: | LI Jing WU Bao yan JIANG Yue rong Life Science College Nankai University Tianjin |
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Abstract: | Objective To explore the possible target and molecular mechanism of Radix Paeoniae 801 (RP801), an effective ingredient extracted from Radix Paeoniae, the Chinese herbal medicine for activating blood circulation to remove blood stasis, using experimental in vitro method by directly detecting the interaction between RP801 and endothelin 1 (ET 1). Methods Piezoelectric quartz crystal biosensor, namely, the quartz crystal microbalance (QCM) was used to detect the specific combining between RP801 and ET 1 by binding avidin to the pre activated Au surface of electrode of QCM, followed by immobilizing biotinylated ET 1 to it, and adding RP801, then the binding curve was recorded. PBS washing was applied at the end of every steps of combining reaction for dissociate the non specific absorption. Results Specific combining of RP801 and ET 1 was found. Conclusion ET 1 could possibly be one of the acting targets of RP801 in the body, that is, RP801 could combine with ET 1 to impede the binding of ET 1 with its receptor, so as to counteract the action of ET 1, dilate blood vessels and inhibit platelet aggregation. |
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Keywords: | Radix Paeoniae 801 endothelin-1 quartz crystal microbalance |
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