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黄芪注射液中黄芪总皂苷的含量测定
引用本文:黄俊斌. 黄芪注射液中黄芪总皂苷的含量测定[J]. 时珍国医国药, 2001, 12(5): 394-395
作者姓名:黄俊斌
作者单位:广东省汕头市中心医院
摘    要:
目的:建立黄芪注射注中黄芪总皂苷含量测定的新方法。方法:采用大孔吸附树脂吸附分离,以5%得草醛冰醋酸溶液和高氯酸为显色剂,比色法测定,测定波长为536nm。结果:在10-50μg.ml^-1浓度范围内,线性关系良好,平均回收率为98.04%,RSD=1.7%。结论:方法简便,结果可靠。

关 键 词:黄芪注射液 黄芪总皂苷 含量 测定 D101大孔树脂 中药
文章编号:1008-0805(2001)05-0394-01
修稿时间:2000-12-26

Content Determination ofThe Total Astragalin in Huangqi Injection
HUANG Jun-bin. Content Determination ofThe Total Astragalin in Huangqi Injection[J]. Lishizhen Medicine and Materia Medica Research, 2001, 12(5): 394-395
Authors:HUANG Jun-bin
Abstract:
Objective: To establish a new method for measuring the content of total astragalin in Huangqi injection. Methods:Total astragalin was seperated by macroporous adsorbe resin, then measured by visible spectrophotometry with 5% veniua-glacial acid solution and perchloric acid as developing olyes and detection wavelegth of 536 nm.Results: The calibration curve was linear in the range of 10~50 μg*ml-1 with r=0.999 8, the average recovery and relative standard diviation were 98.04% and 1.7% respectively. Conclusion: The method is simple. The result is accurate.
Keywords:Huangqi injection  Total astragalin  Determination of content  D 101 macroporous adsorbe resin
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