Action of leukotriene B4 in human granulosa cells in vitro

Leukotriene B4 directly enhanced progesterone release from superfusedhuman granulosa cells. This secretory effect was observed inconcentrations from 10^–12 to 10^–10 M. Lower andhigher concentrations failed to affect progesterone release.When we analysed the high performance liquid chromatographyprofile of supernatant from human granulosa cell cultures, wedetected a leukotriene B4 peak. In conclusion, these data supportthe hypothesis that leukotriene B4 may participate in the intracellularmechanism of progesterone release in human granulosa cells. arachidonate/human granulosa cells/leukotriene B4/5-lipoxygenase/progesterone