Electron-microscopic autoradiography of RNA synthesis in the injured myocardium

A burn of the wall of the left ventricle was produced in newborn rats. Synthesis of RNA in the muscle cells of the heart at a distance from the site of the burn was investigated by electron-microscopic autoradiography 24 h after injury. The tissue was fixed 2 and 6 h after injection of uridine-³H. The density of distribution of silver grains above the nucleus and cytoplasm of the cardiomyocytes was lower in the experimental animals than in the controls.Department of Pathological Anatomy, A. V. Vishnevskii Institute of Surgery, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. V. Smol'yannikov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 6, pp. 751–753, June, 1977.