毛细管电色谱手性配体交换法检测生物样品中的硝基精氨酸异构体研究

目的：建立毛细管电色谱（CEC）分离检测生物样品中硝基精氨酸异构体的方法。方法：采用CEC手性配体交换模式检测血浆样品中D-硝基精氨酸（D—NNA）和L-硝基精氨酸（L—NNA），流动相为50mmol／L醋酸缓冲液[pH5．0，含2mmol／Laspartame，1mmol／L Cu^2＋和5％（v／v）甲醇]；流速为0．02mL／min；操作压为1000psi；检测波长为UV280nm。结果：L—NNA和D—NNA在0．025～0．75mmol／L的浓度范围内峰面积／浓度的相关系数均可达0．99以上，日内变异系数均小于3．0％，日间变异系数分别为3．1％和3．4％。结论：本研究方法具有快速、高分辨、检测样品量和流动相用量少等优点。

Determination of NG-nitro-arginine enantiomers in biological samples with chiral ligand exchange method by capillary electrochromatography

AIM: To study the chiral separation of NG-nitro-arginine enantiomers in biological samples by capillary electrochromatography (CEC). METHODS: The separation of D-NNA and L-NNA in plasma was achieved by chiral ligand exchange method via CEC. An aqueous eluent containing aspartame (2 mmol/L) sufficiently mixed with Cu2+ was used. The eluent was monitored at UV 280 nm. RESULTS: The retention times were 16 and 19 min for L-NNA and D-NNA, respectively. The standard curves of D-NNA and L-NNA ranged from 0.025 to 0.75 mmol/L (R2>0.98). The within-day precision of the quality control samples was 3.0% for both L-NNA and D-NNA. The between-day precision was 3.1% for L-NNA and 3.4% for D-NNA, respectively. CONCLUSION: This method was established to determinate D/L-NNA in biological samples with high resolution and efficiency.