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In vitro and in vivo suppression of hepatocellular carcinoma growth by midkine-antisense oligonucleotide-loaded nanoparticles |
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| Authors: | Li-Cheng Dai Xing Yao Xiang Wang Shu-Qiong Niu Lin-Fu Zhou Fang-Fang Fu Shui-Xin Yang Jin-Liang Ping |
| Affiliation: | 1. Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China 2. Department of General Surgery, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China 3. Zhejiang University School of Medicine, Hangzhou 310016, Zhejiang Province, China 4. Department of Pathology, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China |
| Abstract: | AIM: To synthesize antisense oligonucleotides (ASODNs) of midkine (MK), package the ASODNs with nanoparticles, and to inhibit hepatocellular carcinoma (HCC) growth using these nanoparticles.METHODS: HepG2 cell proliferation was analyzed in vitro using the 3-(4,5-dimethythiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2Htetrazolium, inner salt assay. The in vivo activity of nanoparticles delivering the MK-ASODNs was analyzed by histopathological and immunohistochemical staining and quantitative real time polymerase chain reaction (PCR).RESULTS: The in vitro proliferation of HepG2 cells was significantly inhibited by the nanoparticles packaged with MK-ASODNs (NANO-ASODNs). Furthermore, the NANOASODNs significantly inhibited the growth of HCC in the mouse model. |
| Keywords: | Midkine Nanoparticles Hepatocellular carcinoma Inhibition Drug delivery |
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| 点击此处可从《World journal of gastroenterology : WJG》浏览原始摘要信息 | |