| 切应力作用下肾近端小管上皮细胞纤溶酶原激活物(tPA和uPA)表达的变化及意义 |
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| 引用本文： | 蒲丽君,黄颂敏,柳飞. 切应力作用下肾近端小管上皮细胞纤溶酶原激活物(tPA和uPA)表达的变化及意义[J]. 生物医学工程学杂志, 2008, 25(6) |
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| 作者姓名： | 蒲丽君 黄颂敏 柳飞 |
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| 作者单位： | 1. 四川大学,华西医院,肾脏内科,成都,610041;川北医学院,附属医院,心脏内科,南充,637000
2. 四川大学,华西医院,肾脏内科,成都,610041 |
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| 摘 要： | 检测切应力作用下肾近端小管上皮细胞纤溶酶原激活物tPA和uPA mRNA表达的变化,探讨糖尿病肾病早期小管间质细胞外基质重塑的可能机制.用5 dyn/cm2和10 dyn/cm2的切应力处理肾近端小管上皮细胞(NRK-52E),作用时间分别为1、3和6 h,用RT-PCR法检测tPA及uPA mRNA的表达.结果表明:切应力呈大小和时间依赖性下调肾小管上皮细胞tPA及uPA mRNA的表达.在糖尿病肾病早期,高滤过引起的切应力增加可抑制肾近端小管上皮细胞tPA和uPA mRNA表达,导致肾小管间质纤维蛋白溶解活性降低,参与小管间质细胞外基质的重塑.
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| 关 键 词： | 切应力 纤溶酶原激活物 |
Effects of Shear Stress on Expression of Plasminogen Activator(tPA and uPA) in Cultured Kidney Proximal Tubular Epithelial Cells and Its Significance |
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| Pu Lijun,Huang Songmin,Liu Fei. Effects of Shear Stress on Expression of Plasminogen Activator(tPA and uPA) in Cultured Kidney Proximal Tubular Epithelial Cells and Its Significance[J]. Journal of biomedical engineering, 2008, 25(6) |
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| Authors: | Pu Lijun Huang Songmin Liu Fei |
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| Affiliation: | Department of Nephrology;West China Hospital;Sichuan University;Chengdu 610041;China;Department of Cardiacvascular;Affiliated Hospital of North Sichuan Medical College;Nanchong 637000;China |
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| Abstract: | In this study,we assessed the effects of shear stress on the expression of plasminogen activator(tPA and uPA) mRNA in cultured NRK-52E cells(a kidney proximal tubular epithelial cell line of normal rat origin) and investigated the mechanism of tubulointerstitial extracellular matrix(ECM) remodeling in the early stage of diabetic nephropathy(DN).The cultured NRK-52E cells were exposed to shear stress of 5 and 10 dyn/cm2 for 1,3 and 6 hours respectively.Semi-quantity RT-PCR was used to detect the expression o... |
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| Keywords: | tPA uPA |
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