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| 板蓝根药材HPLC指纹图谱研究 | |
| 引用本文： | 林文艳,莫建霞,于荣敏,周长新,赵昱. 板蓝根药材HPLC指纹图谱研究[J]. 中国现代应用药学, 2005, 22(5): 378-380 |
| 作者姓名： | 林文艳 莫建霞 于荣敏 周长新 赵昱 |
| 作者单位： | [1]浙江大学药学院中药与天然药物研究室,浙江杭州310031 [2]暨南大学药学院天然药物化学教研室,广东广州510632 |
| 摘 要： | 目的 研究板蓝根饮片HPLC指纹图谱。方法 应用RP-HPLC法，梯度洗脱分析市售的11批板蓝根饮片的醋酸乙酯提取物，建立板蓝根饮片的指纹图谱。结果 不同来源的板蓝根饮片的醋酸乙酯提取物的色谱图相近，特征共有峰12个，相对峰面积相差很大。结论 建立的方法用于板蓝根药材的鉴别和质量评价简便、准确，为深入研究板蓝根的质量标准提供了实验基础。 |
| 关 键 词： | 板蓝根 高效液相 指纹图谱 |
| 文章编号： | 1007-7693（2005）05-0378-03 |
| 收稿时间： | 2004-07-26 |
| 修稿时间： | 2004-07-26 |
Studies on HPLC fingerprint chromatogram of Radix Isatidis |
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| LIN Wen-yan~(,MO Jian-xia~(,YU Rong-min~(,ZHOU Chang-xin~(,ZHAO Yu~(. Studies on HPLC fingerprint chromatogram of Radix Isatidis[J]. The Chinese Journal of Modern Applied Pharmacy, 2005, 22(5): 378-380 | |
| Authors: | LIN Wen-yan~( MO Jian-xia~( YU Rong-min~( ZHOU Chang-xin~( ZHAO Yu~( |
| Affiliation: | 1. Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China ;2. Natural Products Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China |
| Abstract: | OBJECTIVE To establish a method for HPLC fingerprint chromatogram determination of Radix Isatidis. METHOD RP-HPLC, gradient elution was used to analyse the components of ethyl acetate abstract from the eleven batches of commodity Radix Isatidis and HPLC fingerprint chromatogram was established. RESULT The HPLC chromatogram of commodity Radix Isatidis from the eleven batches were found similar with twelve common characteristic peaks, but the relative areas of the peaks were showed notable different from each other. CONCLUSION This method can be simply and exactly used in identifying and evaluating Radix Isatidis, which provide experimental base for further quality control of it. |
| Keywords: | Radix Isatidis HPLC fingerprint chromatogram |
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