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| 神经降压素对实验性神经细胞内兴奋性氨基酸含量影响的研究 | |
| 引用本文： | 孙晓江 蔡琰. 神经降压素对实验性神经细胞内兴奋性氨基酸含量影响的研究[J]. 上海医学, 1996, 19(3): 132-134 |
| 作者姓名： | 孙晓江 蔡琰 |
| 作者单位： | 上海第二医科大学附属仁济医院神经生物学实验室，承德医学院附属医院神经生物学实验室 |
| 基金项目： | 上海高等院校科学发展基金和河北省自然科学基金 |
| 摘 要： | 本研究采用小鼠神经母细胞瘤细胞（ＮＢＡ２）无血清培养建立神经细胞实验研究模型。以氨基酸自动分析术观察神经降压素（ＮＴ）对实验性神经细胞内氨基酸类神经递质的影响。结果发现：ＮＴ可使细胞内天门冬氨酸（Ａｓｐ）和谷氨酸（Ｇｌｕ）这两种兴奋性氨基酸含量显著降低（Ｐ＜０．０１），而对抑制性氨基酸中的甘氨酸（Ｇｌｙ）和γ－氨基丁酸（ＧＡＢＡ）的影响不大（Ｐ＜０．０５）。结果提示：ＮＴ使兴奋性氨基酸Ａｓｐ和Ｇｌｕ含量降低的作用可能是其延缓实验性神经细胞老化的机制之一。 |
| 关 键 词： | 神经降压素 神经细胞 天门冬氨酸 谷氨酸 |
THE EFFECTS OF NEUROTENSIN AND ARGININE-VASOPRESSIN ON ASPARTIC AND GLUTAMIC ACIDS IN EXPERIMENTAL NEURONS |
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| Sun Xiaojian,et al.. THE EFFECTS OF NEUROTENSIN AND ARGININE-VASOPRESSIN ON ASPARTIC AND GLUTAMIC ACIDS IN EXPERIMENTAL NEURONS[J]. Shanghai Medical Journal, 1996, 19(3): 132-134 | |
| Authors: | Sun Xiaojian et al. |
| Abstract: | erum-free culture of mouse neuroblastoma A2(NBA2)cells was used in the experimen-tal study of neurons. High speed amino acid analyzer was used to observe the effects of neu-rotensin(NT)and on amino acid neurotransmitters contents in experimental neurons. After addition of NT separately into the culture medium,the following results were ob-tained:NT caused a decrease in the levels of aspartic(Asp)and glutamic(Glu)acids(p<0. 01),with no significant influence on GABA and glycin. The results suggested that theseneuropeptides may play an important role in the modulation of Asp and Glu levels in cellsduring experimental aging. |
| Keywords: | NT experimental neuron Asp Glu high speed amino acid analyzer. |
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