重组人生长激素对去势大鼠正畸牙牙周组织改建的影响

目的:探讨重组人生长激素(recombinanthumangrowthhormone,rh-GH)对去势(ovariectomy,OVX)大鼠正畸牙移动后牙周组织细胞变化的影响。方法:30只7周龄SPF级雌性Wistar大鼠随机分为3组:对照组、去势盐水组(OVX-NS组)和去势生长激素组(OVX-GH组)。将去势摘除双侧卵巢和腹部皮下注射rh-GH作为不同处理因素,观察第15天和第30天时3组大鼠正畸牙移动引起牙槽骨受压侧破骨细胞数的变化,并进行组织学观察。用SPSS12.0软件包对数据进行完全随机设计资料的方差分析。结果:对3组大鼠不同时间正畸牙牙槽骨受压侧破骨细胞计数进行比较,发现同一时间3组数据的差异有统计学意义(P<0.05);OVX-NS组比OVX-GH组显著增多(P<0.05),对照组最少;同组相比,第15天处死的大鼠与第30天处死的大鼠其破骨细胞数无显著差异(P>0.05);牙周组织学观察见,OVX-GH组牙周膜的损伤和创伤性炎症较OVX-NS组明显减轻。结论:去势(成年)大鼠腹部皮下注射rh-GH,能够减少其正畸牙移动引起的受压侧破骨细胞数目,同时可促进牙周组织因正畸力所致的病理性变化的恢复,对成年正畸治疗有良好的协同作用。

Effects of Rh-GH on the remodeling of periodontium in the ovariectomized rats after orthodontic tooth movement

PURPOSE: To investigate the effects of the recombinant human growth hormone (rh-GH) on the periodontal cells in the ovariectomized rats after orthodontic tooth movement. METHODS: Thirty seven-week-old SPF female Wistar rats were randomly divided into three groups: the control group, the ovariectomy-saline group (OVX-NS group) and the variectomy-GH group(OVX-GH group). Different treatments, including variectomy and injecting normal saline solution, were adminstrated on the rats of the three groups. The number of osteoclast in the compressed alveolar bone was counted on the fifteenth and thirtieth days. Histological changes were observed. The data was analyzed with ANOVA by SPSS12.0 software package. RESULTS: By ANOVA analysis, there were significant differences in the number of osteoclast between the three groups in the same period. The number of osteoclast in the compressed alveolar bone of OVX-NS group was significantly more than that of the OVX-GH group (P < 0.05), and that of the control group was the least. In the same group, there wasn't significant difference between the rats killed 15 days later and 30 days later (P > 0.05). The trauma and inflammation of periodontal membrane of OVX-GH group had been significantly improved compared with the OVX-NS group. CONCLUSION: It is suggested that injecting rh-GH can decrease the number of osteoclast in the compressed alveolar bone in the adult rats after orthodontic tooth movement. At the same time, it can improve the recovery of pathologic changes of periodontal tissue caused by the orthodontic force. There will be a synergetic effect between adult orthodontic treatment and rh-GH.