苦参素对巨细胞病毒感染小鼠脑组织损害的影响

目的:研究苦参素对巨细胞病毒(cytomegalovirus, CMV)感染乳鼠脑组织病理损伤的影响.方法:乳鼠脑内接种半数感染剂量的CMV,运用光学显微镜和透射电镜观察苦参素对脑组织病理形态的影响.结果:接种CMV后,乳鼠脑组织出现炎性细胞浸润、灶性坏死;脑组织超微结构出现神经细胞核膜凹陷、染色质聚集成团块状、细胞质透明样改变呈显著空泡化、内质网排列不整齐、尼氏小体变小并逐渐消失等.经苦参素注射液50 mg/kg腹腔注射治疗15 d后,上述病理性改变状况比模型组小鼠明显减轻或好转.结论:苦参素对CMV感染后乳鼠脑组织的病理损伤有明显的保护作用.

Effect of oxymatrine on pathological change in brain tissue of newborn mice infected by cytomegalovirus

Objective: To study the effect of oxymatrine on pathological change in brain tissue of newborn mice infected by cytomegalovirus (CMV). Methods: CMV of TCID 50 was inoculated into the brain of the newborn mice, and the morphological change in the brain tissue infected by CMV was observed with light microscope and transmission electron microscope. Results: In the model control group, the results showed that there were some inflammatory cellular infiltration and focal necrosis in the brain tissue of newborn mice infected by CMV. The ultrastructure change in the brain tissue showed that the nuclear membrane of cerebral neurons sunk, the chromatin deformed and fused into masses, the cytoplasm vacuolated, the endoplasmic reticulum disarranged and the Nissl's body was blurred or disappeared. After being treated with oxymatrine (50 mg/kg, ip) for 15 days, those pathological changes of the brain tissue in the newborn mice could be significantly improved.Conclusion:Oxymatrine has an obvious inhibition on CMV in vivo.