半边莲不同组分对内皮细胞内皮素及内皮源一氧化氮合酶代谢的影响

为了探讨半边莲对动脉内皮保护作用的有效成分 ,提取半边莲两种不同组分用于高脂血症大鼠 ,观察对动脉内皮细胞内皮素和内皮源一氧化氮合酶代谢以及对动脉内皮功能和形态的影响。结果发现 ,高脂血症大鼠应用半边莲B0 0 1组分 6 0天后 ,血浆内皮素浓度和动脉内皮细胞内皮素阳性细胞率明显低于高脂未用药组 (P <0 .0 5 ) ,血浆内皮源一氧化氮合酶浓度显著高于高脂未用药组 (P <0 .0 5 ) ,且动脉内皮损伤减轻 ,但血脂无明显变化。应用半边莲A0 0 1组分的高脂血症大鼠血脂浓度、内皮素和内皮源一氧化氮合酶无明显变化。结果提示 ,半边莲B0 0 1使高脂血症时内皮细胞、内皮素合成及释放减少 ,并可促进内皮源一氧化氮合酶的合成 ,缓解高脂血症对血管内皮的持续损伤。

Effects of Different Element from Scute Llaria Barbata on Metabolism of Endothel in and Endothelial Nitric Oxide Synthase in Arterial Endothelium

Aim To investigate the protection of effective e lements from Scute Llaria Barbata on arterial endothelium. Methods The Wistar rats were fed with high cholesterol diet and treated with the A001 eleme nt and the B001 element of S cute Llaria Barbata for 60 days. The levels of the blood total cholesterol (TG) , triglycerides (TG), endothelin 1(ET 1), endothelial nitric oxide synthase (e N OS) and the positive rate of ET in "en face" preparation of arterial endotheli um were detected. Results The concentrations of TC and TG did not reduce in hyperlipidemic rats treated with both B001 and A001 . The blood ET 1, positive rate of ET in arterial endothelium and the morpholog ical injury of the arterial endothelium decreased significantly (P<0.05) and the blood eNOS increased significa ntly (P<0.05) in the hyperlipidemic rats treated with B001. The hyperlipidemic r ats treated with A001 did not show the same changes. Conclusion The B001 is the effective element of Scute Llaria Barbata, which can protect the arterial endot helium and impede the development of atherosclerosis.