胰腺癌p27kip1、视网膜母细胞瘤基因蛋白和增殖细胞核抗原表达及与临床病理关系的研究

目的　探讨细胞周期负性调控因子p2 7kip1,视网膜母细胞瘤 (Rb)基因蛋白和增殖细胞核抗原 (PC NA)在胰腺癌发生、发展中的作用。方法　应用免疫组织化学技术 (SP法 ) ,对 32例胰腺癌及癌旁组织中p2 7kip1、Rb蛋白和PCNA表达进行检测。结果　p2 7kip1蛋白阳性表达率在胰腺癌组织中为 5 6 2 5 %,显著低于癌旁胰腺组织 (84 37%) (P <0 0 5 ) ,并与胰腺癌组织分化程度及淋巴结转移相关 (P <0 0 5 ) ;Rb基因蛋白阳性表达率在胰腺癌组织中为 5 0 0 0 %,显著低于癌旁胰腺组织 (78 13%) (P <0 0 5 ) ;PCNA阳性表达率在胰腺癌组织中为 71 87%,显著高于癌旁胰腺组织 (4 3 75 %) (P <0 0 5 ) ,并与胰腺癌组织分化程度和淋巴结转移均相关(P <0 .0 5 )。结论　p2 7kip1、Rb基因蛋白和PCNA与胰腺癌发生、发展密切相关。

Expression of p27kip1, Rb protein and PCNA and their relations with clinicopathology in human pancreatic cancer

Objectives To investigate the role of p27 kip1 , Rb protein and PCNA in the occurrence and progress of pancreatic cancer.Methods The expression of p27 kip1 , Rb protein and PCNA in tumor tissue and adjacent tissue of 32 patients with pancreatic cancer were studied by SP immunohistochemical technique.Results p27 kip1 protein positive-expression rate in tumor tissue of pancreatic cancer was 56.25% , which was lower than that in adjacent pancreatic tissue (P<0.05), p27 kip1 protein positive-expression correlated significantly with tumor cell differentiation and lymphy node metastasis (P<0.05);Rb gene protein positive-expression rate in tumor tissues was 50%,also lower than that in adjacent pancreatic tissue (P<0.05); PCNA positive-expression rate in tumor tissue was 71.87%, higher than that in adjacent pancreatic tissue (P<0.05), PCNA positive-expression rate correlated significantly with tumor cell differentiation and lymphy node metastasis (P<0.05).Conclusions p27 kip1 , Rb protein and PCNA may play an important role in the genesis and progress of pancreatic cancer.