HPLC法测定复方罗布麻片中绿原酸和蒙花苷的含量

目的建立HPLC法测定复方罗布麻片中绿原酸和蒙花苷的含量。方法采用KromasilC18色谱柱,以乙腈-0.5%磷酸溶液为流动相,进行梯度洗脱,柱温为30℃,绿原酸的紫外检测波长为326nm,蒙花苷的紫外检测波长为334nm,流速为1.0mL·min^-1。结果绿原酸进样量在0.1629～0.8144μg范围内与峰面积呈良好的线性关系,r=0.9998（n=5）,平均回收率为99.24%,RSD为1.27%（n=6）;蒙花苷进样量在0.0782～0.3912μg范围内与峰面积呈良好的线性关系,r=0.9998（n=5）,平均回收率为98.02%,RSD=1.27%（n=6）。结论本法操作简便,切实可行,获得了满意的测定结果。

Determination of Chlorogenic Acid and Buddleodide in Compound Kendir Leaves Tablets by HPLC

Objective To assay the content of chlorogenic acid and buddleodide of Compound Kendir Leaves Tablets by HPLC. Methods With gradient elution,taking use of Kromasil C18 chromatograthic column; the acetonitrile-0.5% phosphate acid acting as the mobile phase; the column temperature was 30℃; the ultraviolet detection wavelength for chlorogenic acid and buddleodide were 326nm and 334nm respectively; the flow rate was 1.0mL·min^-1 Results The progress of chlorogenic acid presented a good linearity with the peak square in range of 0.1629～0.8144μg,r=0.9998（n=5）. The average rate of recovery was 99.24%（n=6）. RSD was 1.27%（n=6）. The progress of buddleodide presented a good linearity with the peak square in range of 0.0782～0.3912μg,r=0.9998（n=5）. The average rate of recovery was 98.02%（n=6）. RSD was 1.27%（n=6）. Conclusion The method is easy and practical,and the result is satisfied.