| 绿色荧光蛋白在大鼠骨骼肌中的表达 |
| |
| 引用本文： | 田竟生,赵春礼,郑少鹏,商永磊,潘海燕. 绿色荧光蛋白在大鼠骨骼肌中的表达[J]. 解剖学报, 1999, 0(2): 161-162,I012 |
| |
| 作者姓名： | 田竟生 赵春礼 郑少鹏 商永磊 潘海燕 |
| |
| 作者单位： | 首都医科大学北京神经科学研究所,北京,100054 |
| |
| 摘 要： | 研究绿色荧光蛋白在哺乳动物细胞保的表达。方法制备了含绿色荧光蛋白基因的重组ＤＮＡ，经脂质体法转染培养的大鼠骨骼肌细胞，用共聚焦显微镜观察ＧＦＰ的表达。结果大约２０％－３０％的骨骼３同细胞呈现不同强度色荧光。结论ＧＦＰ是研究活细胞生命现象的一种新途径。
|
| 关 键 词： | 绿色荧光蛋白 骨骼肌细胞 大鼠 |
GFP EXPRESSION IN MUSCLE CELLS OF RATS |
| |
| Tian Jingsheng,Zhao Chunli,Zheng Shaopeng,Shang Yonglei,Pan Haiyan. GFP EXPRESSION IN MUSCLE CELLS OF RATS[J]. Acta Anatomica Sinica, 1999, 0(2): 161-162,I012 |
| |
| Authors: | Tian Jingsheng Zhao Chunli Zheng Shaopeng Shang Yonglei Pan Haiyan |
| |
| Abstract: | Objective The expression of green fluorescent protein(GFP) in mammalian cells was studied. Methods The recombinant DNA containing GFP gene was prepared and transfected into the rat muscle cells in culture using lipofectin method. The expression product of GFP gene was observed by confocal microscope. Results About 20% 30% of living muscle cells expresses the product and appears different density of green fluorescent under confocal microscope. Conclusion GFP can be used as a new approach for studies of life sciences. |
| |
| Keywords: | GFP Muscle cell Rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
