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| 运动神经元单克隆抗体的制备及初步鉴定 | |
| 引用本文： | 曹承刚 粟俭. 运动神经元单克隆抗体的制备及初步鉴定[J]. 基础医学与临床, 1993, 13(6): 38-42 |
| 作者姓名： | 曹承刚 粟俭 |
| 作者单位： | 中国医学科学院基础医学研究所，北京医科大学药学院，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所 北京 100005，北京 100005，北京 100005，北京 100005，北京 100005，北京 100005 |
| 基金项目： | 美国CMB＃ 89-521基金，国家自然科学基金 |
| 摘 要： | 以猪脊髓前角运动神经细胞制备的匀浆免疫Ｂａｌｂ／ｃ小鼠。按常规方法制备、筛选抗运动神经元单克隆抗体。总计３５株杂交瘤细胞所分泌的ＭｃＡｂ，与大鼠半月神经节细胞免疫组化反应均呈阴性，３０株与脊髓前角运动细胞（Ｍｎ）呈阳性反应、其中５株ＭｃＡｂｓ免疫组化反应特异性较强，用ＥＬＩＳＡ方法测定其滴度在２０４８～４０９６之间，免疫双扩散法测定，５株杂交瘤均分泌ＩｇＧ。 |
| 关 键 词： | 抗运动神经元 单克隆抗体 制备 |
Preparation and Identification of Monoclonal Antibodies Against Motoneurons |
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| Cao Chenggang Su Jian Zhu Yufan Wang Yun Li Gang Shi Hui Wan Xuancai. Preparation and Identification of Monoclonal Antibodies Against Motoneurons[J]. Basic Medical Sciences and Clinics, 1993, 13(6): 38-42 | |
| Authors: | Cao Chenggang Su Jian Zhu Yufan Wang Yun Li Gang Shi Hui Wan Xuancai |
| Abstract: | In older to obtain selective markers for subpopulation of moto-naurons, 35 monoclonal antibodies were prepared by using mice immunized with homogenates of ventral horn neurons of swine spinal cord.Monoclonal antibodies were screened with immuno-ohistochemistry, Westein blotting, ELISA methods. All of 35 McAb were not reacted with trigeminal ganglion neurons. 30 McAbs were presented positive reaction with ventral horn motoneurons of rat spinal coid. Five them (No. 24B0, 36F6, 18E11, 8B9 and 5B5) exhibited highly specific irnmunohistochemical reaction. They labeled, respectively, the cell bodies of somatic motoneurons (24B0) , somatic and visceral motoneu-rons (18E11, 36F6) 5 somatic, visceral and specific visceral motoneurons(5B5,8B9). 5 McAbs described above are IgGs. |
| Keywords: | monoclonal antibodies against moto-neurons moto-ne rons hypoglossal nerve nucleus |
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