|
|||||
|
|
Herpes simplex virus DNA isolation from infected cells with a novel procedure. |
|
| Authors: | P F Pignatti E Cassai G Meneguzzi N Chenciner G Milanesi |
| Affiliation: | 1. Istituto di Genetica and Laboratorio di Genetica Biochimica ed Evoluzionistica del C.N.R., Pavia, Italy;2. Istituto di Microbiologia, Università di Ferrara, Ferrara, Italy;3. Centre de Biochimie, Faculté des Sciences, Nice, France |
| Abstract: | Herpes Simplex Virus type 1-infected cells were extracted in the presence of 0.25% Triton X-100-0.2 M NaCl. Viral DNA associated with proteins was found in the supernatant after low-speed centrifugation. Only viral DNA was recovered by this procedure, as shown by CsCl density analysis after deproteinization. Full-length viral DNA molecules were observed in the electron microscope. |
| Keywords: | Reprint requests to Pier Franco Pignatti Istituto di Genetica University di Pavia Via S. Epifanio 14 27100 Pavia Italy. |
| 本文献已被 ScienceDirect 等数据库收录！ | |