纳米ZnO对雄性小鼠的体内作用及对生殖系统的影响

目的纳米颗粒因为较小尺寸、较大的表面积使其具有特殊的活性和内在毒性。文中研究纳米ZnO对雄性小鼠的体内作用,观察其对雄性小鼠生殖系统的影响。方法45只6~7周龄的雄性ICR小鼠随机均分3组,每组15只,纳米ZnO处理组隔天腹腔分别注射200、500mg/kg的纳米ZnO,对照组注射等体积等渗盐水,共5次。停药1周后,测量心脏、肝、肾、脾、睾丸和附睾的器官系数,血清生化指标、睾酮和雌二醇水平;显微镜下观察附睾精子数量、活率、畸形率和睾丸内精子计数;主要器官做病理切片和HE染色;电子显微镜观察肝和睾丸内纳米颗粒的分布。TUNEL法检测睾丸生殖细胞凋亡。结果对照组和纳米ZnO200mg/kg组小鼠无死亡,500mg/kg组小鼠死亡2只。与对照组相比,纳米ZnO200mg/kg组器官质量系数均无明显改变(P>0.05);500mg/kg组小鼠脾和肾质量系数明显升高(P<0.05)。纳米ZnO200mg/kg组丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、胆碱(CHO)、尿酸(UA)、肌酸激酶(CK)显著性改变,P<0.05;500mg/kg组肝功能酶ALT、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、T...

Study on the effect of nanosized titanium dioxide for reproductive system of male mice

Objective While research on the potential toxic properties of nanomaterials is now increasing, the in vivo effects of zinc oxide (ZnO) on the main organs were emergent to know. In this study, the effects of nanometer ZnO on male mice, particularly the reproductive system were evaluated. Methods Forty-five male ICR mice, aged 6-7weeks, were randomly divided into control group and two experimental groups which were intraperitoneally injected with 200 (n=15)and 500 mg/kg ZnO(n=15) every other day for 5 times. ...