The effect of l-arginine supplementation and surgical trauma on the frequency of myeloid-derived suppressor cells and T lymphocytes in tumour and blood of colorectal cancer patients |
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| Affiliation: | 1. Division of Oncological Propedeutics, Faculty of Health Sciences, Medical University of Gdansk, Gdynia, Poland;2. Department of Surgical Oncology, Gdynia Oncology Centre, Maritime Polish Red Cross Memorial Hospital, Gdynia, Poland;3. Department of Clinical Nutrition, Medical University of Gdansk, Gdansk, Poland;4. Department of Histology, Medical University of Gdansk, Gdansk, Poland;5. Faculty of Finance and Management, WSB University in Gdansk, Gdansk, Poland;1. Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Shandong, Dezhou, China;2. Dezhou 2nd People''s Hospital, Shandong, Dezhou, China;1. Department of Nephrology, Dialysis and Internal Diseases, Medical University of Warsaw, Warsaw, Poland;2. Department of Anaesthesiology and Intensive Care, Medical University of Warsaw, Warsaw, Poland;3. Department of General, Liver and Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland;4. Department of Medical Informatics and Telemedicine, Medical University of Warsaw, Warsaw, Poland;1. Endocrinology Outpatient Clinic, Central Teaching Hospital, Lodz, Poland;2. Department of Clinical Endocrinology, Medical University of Lodz, Lodz, Poland;1. Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, Lodz, Poland;2. Department of Rheumatology, Medical University of Lodz, Lodz, Poland;1. Department of Pediatric Surgery and Urology, Medical University of Bialystok, Bialystok, Poland;2. Department of Neurosurgery, Medical University of Bialystok, Bialystok, Poland;3. Department of Biophysics, Medical University of Bialystok, Bialystok, Poland;4. Department of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland;5. Department of Invasive Neurology, Medical University of Bialystok, Bialystok, Poland;1. 2nd Department of Nephrology and Hypertension with Dialysis Unit, Medical University of Bialystok, Bialystok, Poland;2. Department of Internal Medicine and Hypertension, Medical University od Bialystok, Bialystok, Poland |
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| Abstract: | Purposel-arginine (L-arg) deficiency causes immunosuppression, but it is unknown if L-arg supplementation in colorectal cancer (CRC) patients restores immune system activity. Our objective was to investigate the effect of L-arg supplementation on the frequency of monocytic (M) and polymorphonuclear (PNM) myeloid-derived suppressor cells (M-MDSCs and PMN-MDSCs, respectively).MethodsWe enrolled 65 CRC patients (34 males, 31 females) aged 69 ?± ?10 years into a prospective, randomised, double-blind study. Twenty-eight patients received L-arg and 37 received placebo for 9 days at a dose of 10 ?g/day. The frequency changes in MDSC, CD4+ cells and the concentration of C-reactive protein (CRP) were assessed before supplementation with L-arg (test 1), after 9 days of supplementation (test 2), and after surgery on day 11 (test 3).ResultsThe frequency of M-MDSC in the tumours of patients receiving L-arg supplementation was higher than in placebo-treated patients, as was the frequency of PMN-MDSC and M-MDSC in the mucosa. CRP concentration in the serum of placebo-treated patients in test 2 was higher than in test 1, and the concentration in the serum of patients with L-arg supplementation in test 2 was lower than in test 1. Moreover, the expression pattern of the argininosuccinate synthase 1 (ASS1) suggests that CRC is not auxotrophic for L-arg.ConclusionsThe results of this study do not support the hypothesis that L-arg supplementation in CRC patients can reduce immunosuppression by decreasing the frequency of suppressor cells and increasing the frequency of effector CD4+ T cells. |
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| Keywords: | Myeloid-derived suppressor cells Non-auxotrophic tumours Colorectal cancer |
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