血清血管内皮生长因子/内皮抑素比值与儿童急性白血病的相关性

目的：检测急性白血病（AL）患儿血清血管内皮生长因子（VEGF）、内皮抑素（ES）水平，探讨VEGF /ES (V/E) 比值与儿童AL的临床相关性。方法：采用酶联免疫双抗体夹心法检测35例AL患儿化疗前后血清VEGF、ES水平及V/E比值，并与30例健康儿童进行比较。结果：化疗前AL患儿血清中VEGF和ES水平分别为196±66 pg/mL和 31±7 ng/mL，显著高于对照组（29±10 pg/mL、19±4 ng/mL; P0.05）。35例AL患儿化疗前血清VEGF、V/E 比值与骨髓白血病细胞数呈正相关 (r=0.301和0.411，P<0.05)。结论：血清VEGF和ES与儿童AL的发生发展有关，V/E比值能更准确反映AL患儿疾病动态变化情况，有利于指导相关治疗。

Correlation between the ratio of serum vascular endothelial growth factor/endostatin and childhood acute leukemia

Objective To investigate the correlation between the ratio of serum vascular endothelial growth factor(VEGF)/endostatin(ES) and childhood acute leukemia(AL).Methods Serum levels of VEGF and ES were measured using ELISA in 35 children with acute AL before and after chemotherapy.The ratio of VEGF/ES was calculated.Thirty healthy children served as the control group.Results The serum levels of VEGF(196±66 pg/mL vs 29±10 pg/mL) and ES(35±7 ng/mL vs 19±4 ng/mL) in the AL group before chemotherapy were significantly higher than those in the control group(P<0.01).The ratio of VEGF/ES in the AL group before chemotherapy was significantly higher than that in the control group(6.7±3.0 vs 1.6±0.7;P<0.01).In 20 children with AL who achieved complete remission,the serum levels of VEGF and ES and the VEGF/ES ratio were reduced after chemotherapy(83±35 pg/mL,27±5 ng/mL,3.1±1.3,respectively;P<0.01),although the serum levels of VEGF and ES were still higher than those in the control group(P<0.01).The VEGF/ES ratio in CR patients was not significantly different from that in the control group.The serum levels of VEGF(r=0.301,P=0.045) and the VEGF/ES ratio(r=0.411,P=0.015) were positively correlated with the count of blast cells in juvenile bone marrow in 35 children with AL before chemotherapy.Conclusions Serum VEGF and ES levels are associated with the development of childhood AL.The VEGF/ES ratio can be used to evaluate the disease progression in children with AL.